Duration effects of alcohol graded concentrations on the extent of lipid peroxidation, testis morphology and sperm quality assessment in Wistar rats

Dorcas Ibukun Akinloye; Regina Ngozi Ugbaja; Adekunle Adeniyi Adebiyi; Olusegun Mark Obafemi Idowu

doi:10.1016/j.toxrep.2022.05.003

Duration effects of alcohol graded concentrations on the extent of lipid peroxidation, testis morphology and sperm quality assessment in Wistar rats

Toxicol Rep. 2022 May 5:9:1082-1091. doi: 10.1016/j.toxrep.2022.05.003. eCollection 2022.

Authors

Dorcas Ibukun Akinloye¹, Regina Ngozi Ugbaja¹, Adekunle Adeniyi Adebiyi², Olusegun Mark Obafemi Idowu³

Affiliations

¹ Department of Biochemistry, College of Biosciences, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Nigeria.
² Department of Veterinary Pathology, College of Veterinary Medicine, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Nigeria.
³ Department of Animal Nutrition, College of Animal Sciences and Livestock Production, Federal University of Agriculture, P.M.B. 2240, Abeokuta, Nigeria.

Abstract

Alcohol consumption is known to cause an array of alcohol-induced biochemical changes in a biological system. This study investigated the durations effects of different alcohol concentrations (30%, 40%, and 50%) on malondialdehyde levels, testes histology, and sperm characteristics in matured male Wistar rats. The rats were divided into four groups namely thus; control, 30%, 40% and 50%. Control group was orally administered 0% alcohol while, group 30%, 40% and 50% received orally 30%, 40% and 50% of alcohol concentrations (3.20 g/ Kg body weight) respectively for maximum durations of 28 days. On the day 1, 7, 14, 21, and 28, five rats from each group (control, 30%, 40% and 50% alcohol) were sacrificed, and malondialdehyde levels, testes histology, and sperm characteristics were examined. Graded alcohol concentrations caused different detrimental effects on sperm characteristics and induced pathological lesions in the testes. Significant increases in serum, liver and testes malondialdehyde levels were durations independent but almost entirely concentrations dependent. Ultimately, administration of alcohol graded concentration led to loss of sperm motility and testicular degeneration in concentration and durations dependent manner without a concomitant increase in the malondialdehyde levels.

Keywords: %, percentage; Alcohol concentrations; C, control administered distilled water; Durations; F, administered 40% ethanol; FUNAAB, Federal University of Agriculture, Abeokuta; MDA levels; MDA, malondialdehyde; N, Normal tissue; PUFAS, polyunsaturated fatty acids; Sperm motility; T, administered 30% ethanol; TBARS, thiobarbituric acid reactive substance; TD, Tissue Damage; Testicular degeneration; V, administered 50% ethanol; g/Kg, gram per kilogram; nmole/L, nanomole per litre.