Scale-up of hydrothermal processing: Liquid hot water and pilot-scale tubular steam explosion batch reactor for bioethanol production using macroalgae Sargassum spp biomass

K D González-Gloria; Rosa M Rodríguez-Jasso; Gilver Rosero-Chasoy; Shiva; Emily T Kostas; E Aparicio; Arturo Sanchez; Iosvany López-Sandin; Héctor A Ruiz

doi:10.1016/j.biortech.2022.128448

Scale-up of hydrothermal processing: Liquid hot water and pilot-scale tubular steam explosion batch reactor for bioethanol production using macroalgae Sargassum spp biomass

Bioresour Technol. 2023 Feb:369:128448. doi: 10.1016/j.biortech.2022.128448. Epub 2022 Dec 10.

Authors

K D González-Gloria¹, Rosa M Rodríguez-Jasso¹, Gilver Rosero-Chasoy¹, Shiva¹, Emily T Kostas², E Aparicio¹, Arturo Sanchez³, Iosvany López-Sandin¹, Héctor A Ruiz⁴

Affiliations

¹ Biorefinery Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo, 25280, Coahuila, Mexico.
² The Advanced Centre of Biochemical Engineering, Department of Biochemical Engineering, University College London, Gower Street, London, WC1E 6BT, UK.
³ Laboratorio de Futuros en Bioenergía, Unidad Guadalajara de Ingeniería Avanzada, Centro de Investigación y Estudios Avanzados (CINVESTAV), Zapopan, Jalisco, 45019, Mexico.
⁴ Biorefinery Group, Food Research Department, School of Chemistry, Autonomous University of Coahuila, Saltillo, 25280, Coahuila, Mexico. Electronic address: hector_ruiz_leza@uadec.edu.mx.

PMID: 36513304
DOI: 10.1016/j.biortech.2022.128448

Abstract

Sargassum spp. is a biomass that can potentially use as an alternative for bioethanol production. Hydrothermal processes (liquid hot water and steam explosion pretreatment) were carried out at different operational conditions. Enzymatic hydrolysis performed a preliminary test with different ratios 1:1 and 1:2 (cellulases and hemicellulases) of enzyme loading, once selected 1:2 ratio was obtained conversion yield of 99.91% and therefore carried a scale-up in stirred bioreactor getting 95.92% saccharification yield. Pre-simultaneous saccharification and fermentation strategy was performed in a continuous stirred tank bioreactor (CSTBR), producing ethanol yield of 57.69%, and for simultaneous saccharification and fermentation strategy was performed in a bubble column reactor was 71.37% ethanol yield. The energy efficiency was analyzed in different scenarios; the best data was 30.19 (g_sugar/MJ) in the bioreactor enzymatic hydrolysis process. This development allows for establishing the conditions for a third-generation biorefinery on a circular bioeconomy using Sargassum biomass.

Keywords: Biofuels; Biorefinery; Enzymes; Fermentation; High-Pressure Technology.

MeSH terms

Biofuels
Biomass
Ethanol
Fermentation
Hydrolysis
Sargassum*
Seaweed*
Steam
Water

Substances

Steam
Water
Ethanol
Biofuels