The scientific community has shown a great deal of interest in sodium lauroyl glycine (SLG) and sodium lauroyl lactylate (SLL), two sustainable and eco-friendly substances that are considered as potential bio-friendly alternatives for petrochemical-based amphiphiles. In the present work, the formation of mixed micelle for SLG and SLL surfactant in water was investigated. Meanwhile, the surface interaction and thermodynamic parameters were calculated according to the surface tension curves. The results indicated that at certain ratios, SLG/SLL surfactant mixtures had synergistic effects that could yield higher surface activity and improve application performance. When the mole fraction of SLL (αSLL) was 0.4, γcmc achieved a minimum of 22.6 mN m-1 and displayed the best foaming properties. The mixed solution exhibited the best wetting ability when αSLL was 0.6. While αSLL was 0.8, the mixed solution showed the optimum dynamic adsorption properties. And it was found that the antibacterial property of SLG and SLL could be partially preserved after compounding. These results demonstrated for the first time that the mixed environmentally friendly surfactant SLG and SLL has a promising prospect for use in the personal care, detergent, and cosmetic industries.