Analysis of Golgi Secretory Functions in Cancer

Priyam Banerjee; Xiaochao Tan; William K Russell; Jonathan M Kurie

doi:10.1007/978-1-0716-2639-9_47

Analysis of Golgi Secretory Functions in Cancer

Methods Mol Biol. 2023:2557:785-810. doi: 10.1007/978-1-0716-2639-9_47.

Authors

Priyam Banerjee¹, Xiaochao Tan², William K Russell³, Jonathan M Kurie⁴

Affiliations

¹ Frits and Rita Markus Bio-Imaging Resource Center, The Rockefeller University, New York, NY, USA.
² Department of Thoracic/Head and Neck Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
³ Department of Biochemistry and Molecular Biology, The University of Texas Medical Branch, Galveston, TX, USA.
⁴ Department of Thoracic/Head and Neck Medical Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA. jkurie@mdanderson.org.

PMID: 36512251
DOI: 10.1007/978-1-0716-2639-9_47

Abstract

Cancer cells utilize secretory pathways for paracrine signaling and extracellular matrix remodeling to facilitate directional cell migration, invasion, and metastasis. The Golgi apparatus is a central secretory signaling hub that is often deregulated in cancer. Here we described technologies that utilize microscopic, biochemical, and proteomic approaches to analyze Golgi secretory functions in genetically heterogeneous cancer cell lines.

Keywords: Cancer; Confocal microscopy; Golgi apparatus; Live-cell imaging; Proteomics; Quantitative image analysis; Secretion; Subcellular fractionation; Vesicle trafficking.

MeSH terms

Golgi Apparatus / metabolism
Humans
Neoplasms* / pathology
Proteomics*
Secretory Pathway
Signal Transduction