Soil organic carbon regulates CH4 production through methanogenic evenness and available phosphorus under different straw managements

Abstract

Methane (CH₄) is the main greenhouse gas emitted from rice paddy fields driven by methanogens, for which methanogenic abundance on CH₄ production has been intensively investigated. However, information is limited about the relationship between methanogenic diversity (e.g., richness and evenness) and CH₄ production. Three independent field experiments with different straw managements including returning method, burial depth, and burial amount were used to identify the effects of methanogenic diversity on CH₄ production, and its regulating factors from soil properties in a rice-wheat cropping system. The results showed that methanogenic evenness (dominance) can explain 23% of variations in CH₄ production potential. CH₄ production potential was positively related to methanogenic evenness (R² = 0.310, p < 0.001), which is driven by soil organic carbon (SOC), available phosphorus (AP), and nitrate (NO₃^-) through structure equation model (SEM). These findings indicate that methanogenic evenness has a critical role in evaluating the responses of CH₄ production to agricultural practices following changes in soil properties. The SEM also revealed that SOC concentration influenced CH₄ production potential indirectly via complementarity of methanogenic evenness (dominance) and available phosphorus (AP). Increasing SOC accumulation improved AP release and stimulated CH₄ production when SOC was at a low level, whereas decreased evenness and suppressed CH₄ production when SOC was at a high level. A nonlinear relationship was detected between SOC and CH₄ production potential, and CH₄ production potential decreased when SOC was ≥14.16 g kg^-1. Our results indicated that the higher SOC sequestration can not only mitigate CO₂ emissions directly but CH₄ emissions indirectly, highlighting the importance to enhance SOC sequestration using optimum agricultural practices in a rice-wheat cropping system.

Keywords: Available phosphorus; Methane; Methanogenic evenness; Soil organic carbon; Straw managements.