Single-Cell Transcriptome Analysis Reveals Paraspeckles Expression in Osteosarcoma Tissues

Emel Rothzerg; Wenyu Feng; Dezhi Song; Hengyuan Li; Qingjun Wei; Archa Fox; David Wood; Jiake Xu; Yun Liu

doi:10.1177/11769351221140101

Single-Cell Transcriptome Analysis Reveals Paraspeckles Expression in Osteosarcoma Tissues

Cancer Inform. 2022 Dec 5:21:11769351221140101. doi: 10.1177/11769351221140101. eCollection 2022.

Authors

Emel Rothzerg^{1

2}, Wenyu Feng³, Dezhi Song^{1

4}, Hengyuan Li^{1

5}, Qingjun Wei⁴, Archa Fox⁶, David Wood⁷, Jiake Xu¹, Yun Liu^{1

4}

Affiliations

¹ School of Biomedical Sciences, The University of Western Australia, Perth, WA, Australia.
² Perron Institute for Neurological and Translational Science, Queen Elizabeth II Medical Centre, Nedlands, WA, Australia.
³ Department of Orthopaedics, Second Affiliated Hospital of Guangxi Medical University, Nanning, China.
⁴ Department of Orthopaedics, First Affiliated Hospital of Guangxi Medical University, Nanning, China.
⁵ Department of Orthopedics, Centre for Orthopedic Research, Second Affiliated Hospital, School of Medicine, Orthopedics Research Institute, Zhejiang University, Hangzhou, China.
⁶ School of Human Sciences and Molecular Sciences, The University of Western Australia and Harry Perkins Institute of Medical Research, Centre for Medical Research, The University of Western Australia, Perth, WA, Australia.
⁷ Medical School, The University of Western Australia, Perth, WA, Australia.

Abstract

Nuclear paraspeckles are subnuclear bodies contracted by nuclear-enriched abundant transcript 1 (NEAT1) long non-coding RNA, localised in the interchromatin space of mammalian cell nuclei. Paraspeckles have been critically involved in tumour progression, metastasis and chemoresistance. To this date, there are limited findings to suggest that paraspeckles, NEAT1 and heterogeneous nuclear ribonucleoproteins (hnRNPs) directly or indirectly play roles in osteosarcoma progression. Herein, we analysed NEAT1, paraspeckle proteins (SFPQ, PSPC1 and NONO) and hnRNP members (HNRNPK, HNRNPM, HNRNPR and HNRNPD) gene expression in 6 osteosarcoma tumour tissues using the single-cell RNA-sequencing method. The normalised data highlighted that the paraspeckles transcripts were highly abundant in osteoblastic OS cells, except NEAT1, which was highly expressed in myeloid cell 1 and 2 subpopulations.

Keywords: NEAT1; Osteosarcoma; hnRNP; lncRNA; paraspeckles; single cell RNA sequencing; tumour microenvironment.