Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

Danilo Correddu; Sabrina Helmy Aly; Giovanna Di Nardo; Gianluca Catucci; Cristina Prandi; Marco Blangetti; Chiara Bellomo; Elisabetta Bonometti; Guido Viscardi; Gianfranco Gilardi

doi:10.1039/d2ra06029a

Enhanced and specific epoxidation activity of P450 BM3 mutants for the production of high value terpene derivatives

RSC Adv. 2022 Nov 28;12(52):33964-33969. doi: 10.1039/d2ra06029a. eCollection 2022 Nov 22.

Affiliations

¹ Department of Life Sciences and Systems Biology, University of Torino Via Accademia Albertina 13 10123 Torino Italy gianfranco.gilardi@unito.it.
² Department of Chemistry, University of Torino Via P. Giuria 7 10125 Torino Italy.

Abstract

Terpenes are natural molecules of valuable interest for different industrial applications. Cytochromes P450 enzymes can functionalize terpenoids to form high value oxidized derivatives in a green and sustainable manner, representing a valid alternative to chemical catalysis. In this work, an enhanced and specific epoxidation activity of cytochrome P450 BM3 mutants was found for the terpenes geraniol and linalool. This is the first report showing the epoxidation of linalool by P450 BM3 and its mutant A2 (Asp251Gly/Gln307His) with the formation of valuable oxide derivatives, highlighting the relevance of this enzymes for industrial applications.