Current advances of Pichia pastoris as cell factories for production of recombinant proteins

Yingjie Pan; Jiao Yang; Jianping Wu; Lirong Yang; Hao Fang

doi:10.3389/fmicb.2022.1059777

Current advances of Pichia pastoris as cell factories for production of recombinant proteins

Front Microbiol. 2022 Nov 24:13:1059777. doi: 10.3389/fmicb.2022.1059777. eCollection 2022.

Authors

Yingjie Pan¹, Jiao Yang^{1

2}, Jianping Wu^{1

2}, Lirong Yang^{1

2}, Hao Fang^{1

2

3}

Affiliations

¹ ZJU-Hangzhou Global Scientific and Technological Innovation Center, Zhejiang University, Hangzhou, Zhejiang, China.
² College of Chemical and Biological Engineering, Zhejiang University, Hangzhou, Zhejiang, China.
³ College of Life Sciences, Northwest A&F University, Xianyang, Shaanxi, China.

Abstract

Pichia pastoris (syn. Komagataella spp.) has attracted extensive attention as an efficient platform for recombinant protein (RP) production. For obtaining a higher protein titer, many researchers have put lots of effort into different areas and made some progress. Here, we summarized the most recent advances of the last 5 years to get a better understanding of its future direction of development. The appearance of innovative genetic tools and methodologies like the CRISPR/Cas9 gene-editing system eases the manipulation of gene expression systems and greatly improves the efficiency of exploring gene functions. The integration of novel pathways in microorganisms has raised more ideas of metabolic engineering for enhancing RP production. In addition, some new opportunities for the manufacture of proteins have been created by the application of novel mathematical models coupled with high-throughput screening to have a better overview of bottlenecks in the biosynthetic process.

Keywords: CRISPR/Cas9; Pichia pastoris; metabolic engineering; modeling; recombinant protein.

Publication types

Review

Grants and funding

The financial supports from the National Natural Science Foundation of China (NSFC) (No. 22007079) and the Start-up Fund for Talent Introduction from ZJU-Hangzhou Global Scientific and Technological Innovation Center (K02013020) were acknowledged.