Data-driven discoveries on widespread contamination of freshwater reservoirs by dominant antibiotic resistance genes

Zhao-Feng Guo; Wiebke J Boeing; Yao-Yang Xu; Edoardo Borgomeo; Dong Liu; Yong-Guan Zhu

doi:10.1016/j.watres.2022.119466

Data-driven discoveries on widespread contamination of freshwater reservoirs by dominant antibiotic resistance genes

Water Res. 2023 Feb 1:229:119466. doi: 10.1016/j.watres.2022.119466. Epub 2022 Dec 5.

Authors

Zhao-Feng Guo¹, Wiebke J Boeing², Yao-Yang Xu³, Edoardo Borgomeo⁴, Dong Liu¹, Yong-Guan Zhu⁵

Affiliations

¹ Key Laboratory of Urban Environment and Health, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China; Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, PR China.
² Department of Fish, Wildlife & Conservation Ecology, New Mexico State University, Las Cruces, NM 88003, USA.
³ Key Laboratory of Urban Environment and Health, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, PR China. Electronic address: yyxu@iue.ac.cn.
⁴ Environmental Change Institute, University of Oxford, Oxford OX1 3QY, UK.
⁵ Key Laboratory of Urban Environment and Health, Ningbo Observation and Research Station, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen 361021, PR China; Zhejiang Key Laboratory of Urban Environmental Processes and Pollution Control, CAS Haixi Industrial Technology Innovation Center in Beilun, Ningbo 315830, PR China.

PMID: 36502654
DOI: 10.1016/j.watres.2022.119466

Abstract

The propagation of antibiotic resistance genes (ARGs) in freshwater reservoirs threatens ecosystem security and human health, and has attracted increasing attention. A series of recent research articles on ARGs provides a unique opportunity for data-driven discoveries in this emerging field. Here, we mined data from a total of 290 samples from 60 reservoirs worldwide with a data-driven framework (DD) developed to discover geographical distribution, influencing factors and pollution hotspots of ARGs in freshwater reservoirs. Most data came from Asia and Europe where nine classes of ARGs were most frequently detected in reservoirs with multi-drug resistance and sulfonamide resistance genes prevailing. Factors driving distribution of reservoir ARGs differed between reservoir waters and sediments, and interactions among these factors had linear or nonlinear enhancement effects on the explanatory power of ARG distribution. During the cold season, small-sized reservoir waters rich in organic carbon, mobile genetic elements (MGEs) and antibiotics had a higher pollution potential of ARGs; during the spring drought, sediments in large reservoirs located in densely populated areas were more conducive to dissemination of ARGs due to their richness in antibiotics and MGEs. Thus, distribution pattern of ARG pollution hotspots in reservoir waters and sediments varies greatly depending on the differences of internal and external factors. From the "One Health" perspective, this widespread contamination of freshwater reservoirs by ARGs we discovered through the DD framework should be a push to promote integrated research across regions and disciplines. Especially the human - food-chain - ecosystem interface needs an improved understanding of ARG contamination mechanisms and targeted monitoring and evaluation systems should be developed to maintain all ecosystem services in freshwater reservoirs as well as to safeguard human health.

Keywords: Antibiotic resistance genes; Distribution; Freshwater reservoirs; Influencing factors; Pollution hotspots.

MeSH terms

Anti-Bacterial Agents / pharmacology
Drug Resistance, Microbial / genetics
Ecosystem*
Fresh Water
Genes, Bacterial*
Humans

Substances

Anti-Bacterial Agents