Ameliorative Effect of Quercetin against Abamectin-Induced Hemato-Biochemical Alterations and Hepatorenal Oxidative Damage in Nile Tilapia, Oreochromis niloticus

Abdallah Tageldein Mansour; Heba H Mahboub; Rehab M Amen; Marwa A El-Beltagy; Amany Ramah; Abdelfattah M Abdelfattah; Hossam S El-Beltagi; Tarek A Shalaby; Hesham S Ghazzawy; Khaled M A Ramadan; Adnan H M Alhajji; Heba S Hamed

doi:10.3390/ani12233429

Ameliorative Effect of Quercetin against Abamectin-Induced Hemato-Biochemical Alterations and Hepatorenal Oxidative Damage in Nile Tilapia, Oreochromis niloticus

Animals (Basel). 2022 Dec 5;12(23):3429. doi: 10.3390/ani12233429.

Authors

Affiliations

¹ Fish and Animal Production and Aquaculture Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
² Fish and Animal Production Department, Faculty of Agriculture (Saba Basha), Alexandria University, Alexandria 21531, Egypt.
³ Department of Aquatic Animal Medicine, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
⁴ Department of Zoology, Faculty of Science, Mansoura University, Mansoura 35516, Egypt.
⁵ Biochemistry Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, Egypt.
⁶ Graduate School of Medicine and Veterinary Medicine, University of Miyazaki, 1-1 Gakuen Kibanadai-nishi, Miyazaki 889-2192, Japan.
⁷ Department of Forensic Medicine and Toxicology, Faculty of Veterinary Medicine, Benha University, Qalyubia 13518, Egypt.
⁸ Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City 32897, Egypt.
⁹ Agricultural Biotechnology Department, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
¹⁰ Biochemistry Department, Faculty of Agriculture, Cairo University, Giza 12613, Egypt.
¹¹ Department of Arid Land Agriculture, College of Agricultural and Food Science, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
¹² Date Palm Research Center of Excellence, King Faisal University, Hofuf 31982, Saudi Arabia.
¹³ Central Laboratories, Department of Chemistry, King Faisal University, Al-Ahsa 31982, Saudi Arabia.
¹⁴ Department of Zoology, Faculty of Women for Arts, Science & Education, Ain Shams University, Cairo 11757, Egypt.

Abstract

Abamectin (ABM) is a common agricultural pesticide and veterinary anthelmintic drug. It can discharge from the sites of application to aquatic systems via surface run-off or spray drift, causing harmful effects to aquatic organisms. The present study investigated the protective effect of dietary quercetin supplementation on hemato-biochemical parameters and hepato-renal oxidative stress biomarkers in Nile tilapia (Oreochromis niloticus) exposed to a sublethal dose of ABM. Fish were allocated into six equal groups. The first group was kept as a control group. The second and third groups (Q₄₀₀, and Q₈₀₀) were fed diets supplemented with two quercetin levels (400 and 800 mg/kg diet), respectively. The fourth group (ABM) was intoxicated with 20.73 µg/L of ABM. The fifth and sixth groups (ABM + Q₄₀₀, and ABM + Q₈₀₀) were fed diet supplemented with two quercetin levels (400 and 800 mg/kg diet) and simultaneously intoxicated with ABM for 60 days. The results showed that ABM significantly decreased RBCs, hemoglobin content, hematocrit, total protein, albumin levels, and acetylcholinesterase activity activities compared to the control. Meanwhile, ABM significantly increased white blood cells, glucose, total lipids, cholesterol, and alanine and aspartate aminotransferase activities. Liver and kidney levels of lipid peroxidation was significantly increased, while hepato-renal antioxidant biomarkers (reduced glutathione, super oxide dismutase, catalase, and total antioxidant capacity) were significantly decreased upon ABM exposure. On the other hand, quercetin dietary supplementation improved the hemato-biochemical alterations and alleviated oxidative stress induced by ABM exposure. Fish supplemented with quercetin at a level of 800 mg/kg diet showed better alleviating effects against ABM compared to 400 mg/kg diet. Based on these study findings, we suggest that quercetin dietary supplementation (800 mg/kg) offered direct protection against ABM-induced physiological disturbance and oxidative stress in Nile tilapia.

Keywords: Nile tilapia; hematobiochemical; ivermectin; organ’s dysfunction; oxidative stress; quercetin.

Grants and funding

Grant NO: INST184/Deputyship for Research & Innovation, Ministry of Education in Saudi Arabia