Altered functional connectivity strength in chronic insomnia associated with gut microbiota composition and sleep efficiency

Ziwei Chen; Ying Feng; Shumei Li; Kelei Hua; Shishun Fu; Feng Chen; Huiyu Chen; Liping Pan; Caojun Wu; Guihua Jiang

doi:10.3389/fpsyt.2022.1050403

Altered functional connectivity strength in chronic insomnia associated with gut microbiota composition and sleep efficiency

Front Psychiatry. 2022 Nov 22:13:1050403. doi: 10.3389/fpsyt.2022.1050403. eCollection 2022.

Authors

Ziwei Chen^{1

2}, Ying Feng³, Shumei Li², Kelei Hua², Shishun Fu², Feng Chen², Huiyu Chen^{2

4}, Liping Pan¹, Caojun Wu^{2

4}, Guihua Jiang^{2

1}

Affiliations

¹ Jinan University, Guangzhou, China.
² Department of Medical Imaging, Guangdong Second Provincial General Hospital, Guangzhou, China.
³ Department of Radiology, Affiliated Hospital of Chengdu University, Chengdu, China.
⁴ The Second School of Clinical Medicine, Southern Medical University, Guangzhou, China.

Abstract

Background: There is limited evidence on the link between gut microbiota (GM) and resting-state brain activity in patients with chronic insomnia (CI). This study aimed to explore the alterations in brain functional connectivity strength (FCS) in CI and the potential associations among altered FCS, GM composition, and neuropsychological performance indicators.

Materials and methods: Thirty CI patients and 34 age- and gender-matched healthy controls (HCs) were recruited. Each participant underwent resting-state functional magnetic resonance imaging (rs-fMRI) for the evaluation of brain FCS and was administered sleep-, mood-, and cognitive-related questionnaires for the evaluation of neuropsychological performance. Stool samples of CI patients were collected and subjected to 16S rDNA amplicon sequencing to assess the relative abundance (RA) of GM. Redundancy analysis or canonical correspondence analysis (RDA or CCA, respectively) was used to investigate the relationships between GM composition and neuropsychological performance indicators. Spearman correlation was further performed to analyze the associations among alterations in FCS, GM composition, and neuropsychological performance indicators.

Results: The CI group showed a reduction in FCS in the left superior parietal gyrus (SPG) compared to the HC group. The correlation analysis showed that the FCS in the left SPG was correlated with sleep efficiency and some specific bacterial genera. The results of CCA and RDA showed that 38.21% (RDA) and 24.62% (CCA) of the GM composition variation could be interpreted by neuropsychological performance indicators. Furthermore, we found complex relationships between Alloprevotella, specific members of the family Lachnospiraceae, Faecalicoccus, and the FCS alteration, and neuropsychological performance indicators.

Conclusion: The brain FCS alteration of patients with CI was related to their GM composition and neuropsychological performance indicators, and there was also an association to some extent between the latter two, suggesting a specific interaction pattern among the three aspects: brain FCS alteration, GM composition, and neuropsychological performance indicators.

Keywords: brain function; chronic insomnia; functional connectivity strength; gut microbiota; resting-state fMRI.