Objective: To observe the evaluation value of characteristics of bone marrow cell morphology and immunophenotype in patients with non-Hodgkin lymphoma (NHL) leukemia on the bone marrow invasion of NHL.
Methods: The clinical data of 104 patients with NHL treated in the hospital from March 2016 to March 2021 were retrospectively analyzed, the characteristics of bone marrow smear morphology were recorded, and the analysis of bone marrow immunophenotype was performed, the evaluation value of bone marrow cell morphology and immunophenotype on NHL bone marrow invasion was analyzed.
Results: One hundred and four patients with NHL leukemia were found to have increased lymphoma cells by examination of bone marrow cell morphology, including 57 cases of B-cell type, 39 cases of T-cell type and 8 cases of NK/T cell type. The characteristics of bone marrow cell morphology were as follows: the cell body was large, irregular or round like in shape, the cytoplasm was much and mostly stained blue, a few cells could see a few granules, the nucleus was round or round like, some were twisted, the chromatin was thick and nucleolus was different, of which the T-cell type lymphoma cells had strong heteromorphism and more obvious nucleolus. B-cell type mainly expressed CD19, HLA-DR and CD20, and the positive rate was ≥70%. T-cell type mainly expressed CD7, HLA-DR and CD38, and the positive rate was ≥40%. NK/T cell types mainly expressed CD56 and CD161, and the positive rates was ≥50%. Compared the proportion of lymphoma cells between bone marrow smear and immunophenotype examination, there was no statistical significant difference (P>0.05).
Conclusion: The characteristics of bone marrow cell morphology and immunophenotype have certain application value in the evaluation of bone marrow invasion in patients with NHL leukemia, both can complement each other and provide a feasible mean for the effective evaluation of bone marrow invasion in patients with NHL leukemia.
题目: NHL白血病患者骨髓细胞形态学和免疫分型特征分析及对NHL骨髓侵犯的评估分析.
目的: 观察非霍奇金淋巴瘤（NHL）白血病患者骨髓细胞形态学和免疫分型特征对NHL骨髓侵犯的评估价值.
方法: 回顾性分析2016年3月-2021年3月本院收治的104例NHL白血病患者的临床资料，记录患者骨髓涂片形态学特点，并行骨髓免疫分型分析，分析骨髓细胞形态学和免疫分型对NHL骨髓侵犯的评估价值.
结果: 104例NHL白血病患者经骨髓细胞形态学检查均有淋巴瘤细胞增多，其中B淋巴细胞型57例，T淋巴细胞型39例，NK/T细胞型8例；形态学特点为：淋巴瘤细胞胞体大，形状为不规则形或类圆形，胞浆量多，染色为蓝色，少数细胞可见少许颗粒，核为圆形或类圆形，部分有扭曲，染色质粗，核仁隐显不一，其中T细胞型患者淋巴瘤细胞形态具有较强异形性，核仁更明显。B淋巴细胞型主要表达CD19、HLA-DR、CD20，阳性率均≥70%；T淋巴细胞型主要表达CD7、HLA-DR、CD38，阳性率均≥40%；NK/T细胞型主要表达CD56、CD161，阳性率均≥50%。骨髓涂片与免疫分型检测的骨髓中淋巴瘤细胞比例比较，差异无统计学意义（P>0.05）.
结论: 骨髓细胞形态学和免疫分型特征在评估NHL白血病患者骨髓侵犯中有一定应用价值，二者可相互补充，为NHL白血病患者骨髓侵犯的有效评估提供可行手段.
Keywords: bone marrow invasion; immunophenotype; morphology of bone marrow cell; non-Hodgkin lymphoma leukemia.