Simultaneous removal of nitrate and ammonium by hydrogen-based partial denitrification coupled with anammox in a membrane biofilm reactor

Si Pang; Yun Zhou; Lin Yang; Jingzhou Zhou; Xiaodi Li; Siqing Xia

doi:10.1016/j.biortech.2022.128443

Simultaneous removal of nitrate and ammonium by hydrogen-based partial denitrification coupled with anammox in a membrane biofilm reactor

Bioresour Technol. 2023 Feb:369:128443. doi: 10.1016/j.biortech.2022.128443. Epub 2022 Dec 5.

Authors

Si Pang¹, Yun Zhou², Lin Yang¹, Jingzhou Zhou¹, Xiaodi Li¹, Siqing Xia³

Affiliations

¹ State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China.
² College of Resources and Environment, Huazhong Agricultural University, Wuhan 430070, China.
³ State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, China; Shanghai Institute of Pollution Control and Ecological Security, Shanghai 200092, China. Electronic address: siqingxia@tongji.edu.cn.

PMID: 36470489
DOI: 10.1016/j.biortech.2022.128443

Abstract

Hydrogen-based membrane biofilm reactors (MBfRs) are effective for nitrogen removal. However, the safety of hydrogen limited the application of MBfR. Here, a hydrogen-based partial denitrification system coupled with anammox (H₂-PDA) was constructed in an MBfR for reducing hydrogen demand significantly. The metabolomics and structures of microbial communities were analyzed to determine the phenotypic differences and drivers underlying denitrification, anammox, and H₂-PDA. These findings indicated that total nitrogen (TN) removal increased from 57.1% in S1 to 93.7% in S2. During the H₂-PDA process, partial denitrification and anammox contributed to TN removal by 93.7% and 6.3%, respectively. Community analysis indicated that the H₂-PDA system was dominated by the genus Meiothermus, which is involved in partial denitrification. Collectively, these findings confirmed the feasibility of incorporating the H₂-PDA process in a MBfR and form a foundation for the establishment of novel and practical methods for efficient nitrogen removal.

Keywords: Hydrogen-based partial denitrification coupled with anammox (H(2)-PDA); Membrane biofilm reactor (MBfR); Metabonomics; Microbial community; Nitrate and ammonium removal.

MeSH terms

Ammonium Compounds*
Anaerobic Ammonia Oxidation
Anaerobiosis
Biofilms
Bioreactors
Denitrification
Methane
Nitrates*
Nitrogen
Oxidation-Reduction

Substances

Nitrates
Ammonium Compounds
Methane
Nitrogen