Strategies for genotype-flexible plant transformation

Keunsub Lee; Kan Wang

doi:10.1016/j.copbio.2022.102848

Strategies for genotype-flexible plant transformation

Curr Opin Biotechnol. 2023 Feb:79:102848. doi: 10.1016/j.copbio.2022.102848. Epub 2022 Dec 1.

Authors

Keunsub Lee¹, Kan Wang²

Affiliations

¹ Department of Agronomy, Iowa State University, Ames, IA 50011, USA; Crop Bioengineering Center, Iowa State University, Ames, IA 50011, USA.
² Department of Agronomy, Iowa State University, Ames, IA 50011, USA; Crop Bioengineering Center, Iowa State University, Ames, IA 50011, USA. Electronic address: kanwang@iastate.edu.

PMID: 36463838
DOI: 10.1016/j.copbio.2022.102848

Abstract

Recent advances in the genome-editing tools have demonstrated a great potential for accelerating functional genomics and crop trait improvements, but the low efficiency and genotype dependence in plant transformation hinder practical applications of such revolutionary tools. Morphogenic transcription factors (MTFs) such as Baby boom, Wuschel2, GROWTH-REGULATING FACTOR5, GROWTH-REGULATING FACTOR4 and its cofactor GRF-INTERACTING FACTOR1, and Wuschel-homeobox 5 related have been shown to greatly enhance plant transformation efficiency and expand the range of amenable species and genotypes. This review will summarize recent advancements in plant transformation technologies with an emphasis on the strategies developed for genotype-flexible transformation methods utilizing MTFs for both monocots and dicot plant species. We highlight several breakthrough studies that demonstrated a wide range of applicability.

Publication types

Review
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Crops, Agricultural / genetics
Gene Editing*
Genome, Plant
Genomics* / methods
Genotype
Plant Breeding
Plants, Genetically Modified / genetics
Transcription Factors / genetics

Substances

Transcription Factors