Soybean meal peptides regulated membrane phase of giant unilamellar vesicles: A key role for bilayer amphipathic region localization

Feng Jiang; Jingbo Liu; Zhiyang Du; Xuanting Liu; Xiaomin Shang; Yiding Yu; Ting Zhang

doi:10.1016/j.foodres.2022.111924

Soybean meal peptides regulated membrane phase of giant unilamellar vesicles: A key role for bilayer amphipathic region localization

Food Res Int. 2022 Dec;162(Pt A):111924. doi: 10.1016/j.foodres.2022.111924. Epub 2022 Sep 13.

Authors

Feng Jiang¹, Jingbo Liu², Zhiyang Du³, Xuanting Liu⁴, Xiaomin Shang⁵, Yiding Yu⁶, Ting Zhang⁷

Affiliations

¹ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: jiangfeng18@mails.jlu.edu.cn.
² Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: ljb168@sohu.com.
³ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: dzy2635@163.com.
⁴ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: lxt920523@163.com.
⁵ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: xmshang@jlu.edu.cn.
⁶ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: yuyiding1989@hotmail.com.
⁷ Jilin Provincial Key Laboratory of Nutrition and Functional Food and College of Food Science and Engineering, Jilin University, Changchun 130062, People's Republic of China. Electronic address: tingzhang413@hotmail.com.

PMID: 36461188
DOI: 10.1016/j.foodres.2022.111924

Abstract

Membrane phase separation forms liquid-ordered (Lo) and liquid-disordered (Ld) phases and is involved in cellular processes and functions. Our previous study has confirmed that peptides can regulate phase separation by increasing the Lo phase. However, the specific mechanisms underlying the phase separation regulation of peptides remain poorly understood. This study aimed to explore the effect of soybean meal peptides on phase separation and illustrate the correlation between phase regulation and membrane localization of the peptides. Phase separation was studied by giant unilamellar vesicles (GUVs), and membrane localization of the peptides was detected by steady-state fluorescence quenching. Our results revealed that peptides YYK, CLA, and SLW enhanced the Lo phase while WLQ decreased the Lo phase. The localization in the membrane amphiphilic region of the peptides played a crucial role in their regulation of phase separation. The more localization of the peptides (YYK, CLA, and SLW) in the membrane amphiphilic region, the stronger the capacity to increase the Lo phase.

Keywords: Bilayer localization; Giant unilamellar vesicles; Membrane; Peptides; Phase separation; Soybean meal.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Fabaceae*
Glycine max*
Membranes
Peptides
Unilamellar Liposomes

Substances

Unilamellar Liposomes
Peptides