Integrative application of heavy metal-resistant bacteria, moringa extracts, and nano-silicon improves spinach yield and declines its contaminant contents on a heavy metal-contaminated soil

Abdelsatar M A E Eltahawy; El-Sayed A M Awad; Ahmed H Ibrahim; Abdel-Rahman M A Merwad; El-Sayed M Desoky

doi:10.3389/fpls.2022.1019014

Integrative application of heavy metal-resistant bacteria, moringa extracts, and nano-silicon improves spinach yield and declines its contaminant contents on a heavy metal-contaminated soil

Front Plant Sci. 2022 Nov 15:13:1019014. doi: 10.3389/fpls.2022.1019014. eCollection 2022.

Authors

Abdelsatar M A E Eltahawy¹, El-Sayed A M Awad¹, Ahmed H Ibrahim¹, Abdel-Rahman M A Merwad¹, El-Sayed M Desoky²

Affiliations

¹ Soil Science Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.
² Botany Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt.

Abstract

Microorganism-related technologies are alternative and traditional methods of metal recovery or removal. We identified and described heavy metal-resistant bacteria isolated from polluted industrial soils collected from various sites at a depth of 0-200 mm. A total of 135 isolates were screened from polluted industrial soil. The three most abundant isolate strains resistant to heavy metals were selected: Paenibacillus jamilae DSM 13815T DSM (LA22), Bacillus subtilis ssp. spizizenii DSM 15029T DSM (MA3), and Pseudomonas aeruginosa A07_08_Pudu FLR (SN36). A test was conducted to evaluate the effect of (1) isolated heavy metal-resistant bacteria (soil application), (2) a foliar spray with silicon dioxide nanoparticles (Si-NPs), and (3) moringa leaf extract (MLE) on the production, antioxidant defense, and physio-biochemical characteristics of spinach grown on heavy metal-contaminated soil. Bacteria and MLE or Si-NPs have been applied in single or combined treatments. It was revealed that single or combined additions significantly increased plant height, shoot dry and fresh weight, leaf area, number of leaves in the plant, photosynthetic pigments content, total soluble sugars, free proline, membrane stability index, ascorbic acid, relative water content, α-tocopherol, glycine betaine, glutathione, and antioxidant enzyme activities (i.e., peroxidase, glutathione reductase, catalase, superoxide dismutase, and ascorbate peroxidase) compared with the control treatment. However, applying bacteria or foliar spray with MLE or Si-NPs significantly decreased the content of contaminants in plant leaves (e.g., Fe, Mn, Zn, Pb, Cd, Ni, and Cu), malondialdehyde, electrolyte leakage, superoxide radical $(O_{2}^{\cdot -})$ , and hydrogen peroxide (H₂O₂). Integrative additions had a more significant effect than single applications. It was suggested in our study that the integrative addition of B. subtilis and MLE as a soil application and as a foliar spray, respectively, is a critical approach to increasing spinach plant performance and reducing its contaminant content under contaminated soil conditions.

Keywords: antioxidant; chlorophyll; heavy metal; identification; moringa leaf extract; spinach.