The coexistence of copper ions and TC affected the binding ability and the reaction order between extracellular polymeric substances of aerobic granular sludge and exogenous substances

Abstract

As a barrier against external toxic effects, extracellular polymeric substances (EPSs) directly affect the toxicity and removal efficiency of exogenous substances. The reaction of EPSs with exogenous substances has been taken into consideration. The contents of EPSs in sludge cultivated by different influent water vary greatly, which leads to great differences in the binding ability and reaction sequence between EPSs and exogenous substances. However, the results in this respect are very limited. In this study, the binding characteristics between exogenous tetracycline (TC)/copper ions (Cu²⁺) and EPSs from aerobic granular sludge cultured under single and coexisting TC/Cu²⁺ were assessed by three-dimensional fluorescence-parallel factor analysis. The pollutants in the influent water could directionally induce microorganisms to secrete more EPSs, while fluorescence substances in EPSs could combine with the exogenous substances to lessen their effects. In the presence of coexisting TC and Cu²⁺ in the influent water, the ability of fluorescence substances in EPSs to combine with exogenous TC or Cu²⁺ weakened, and humic substances in EPSs were more susceptible than protein substances to binding with exogenous substances. However, the reaction order between EPSs components and exogenous TC or Cu²⁺ was opposite, and the ability of fluorescence substances in EPSs to combine with exogenous TC or Cu²⁺ was enhanced under individual TC or Cu²⁺ existing in the influent water. This study provided new insights into the interaction between EPSs and exogenous substances.

Keywords: Cu2+; Extracellular polymeric substances; Parallel factor analysis; Tetracycline; Two-dimensional correlation spectroscopy.