In order to better study the interaction between epidemic propagation and information diffusion, a new coupling model on multiplex networks with time delay is put forward in this paper. One layer represents the information diffusion about epidemics. There is not only information about the positive prevention of infectious diseases but also negative preventive information. Meanwhile, the dissemination of information at this layer will be influenced by the mass media, which can convey positive and reliable preventive measures to help the public avoid exposure to contagion. The other layer represents the transmission of infectious diseases, and the public in this layer no longer only exchange information related to infectious diseases in the virtual social network like the information layer but spread infectious diseases through contact among people. The classical SIR model is used to model for epidemic propagation. Since each infected individual needs to spend enough time to recover, the infected one at one time does not necessarily change to the recovered one at the next time, so time delay is an essential factor to be considered in the model. Based on the microscopic Markov chain approach, this paper obtains an explicit expression for epidemic threshold in the two-layered multiplex networks with time delay, which reveals some main factors affecting epidemic threshold. In particular, the time delay has a noticeable effect on the epidemic threshold to some extent. Finally, the influence of these main factors on the epidemic threshold and their interaction are proved through numerical simulations.