Transcriptomic insights into the effects of abscisic acid on the germination of Magnolia sieboldii K. Koch seed

Xiu-Jun Lu; Wan-Qi Zeng; Lei Wang; Xiao-Lin Zhang

doi:10.1016/j.gene.2022.147066

Transcriptomic insights into the effects of abscisic acid on the germination of Magnolia sieboldii K. Koch seed

Gene. 2023 Feb 15:853:147066. doi: 10.1016/j.gene.2022.147066. Epub 2022 Nov 29.

Authors

Xiu-Jun Lu¹, Wan-Qi Zeng¹, Lei Wang¹, Xiao-Lin Zhang²

Affiliations

¹ Department of Forestry, Shenyang Agricultural University, Shenyang 110866, China.
² Department of Forestry, Shenyang Agricultural University, Shenyang 110866, China; State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing 100091, China. Electronic address: zxlsyau@syau.edu.cn.

PMID: 36455787
DOI: 10.1016/j.gene.2022.147066

Abstract

Magnolia sieboldii K. Koch is a deciduous tree species. However, the wild resource of M. sieboldii has been declining due to excessive utilization and seed dormancy. In our previous research, M. sieboldii seeds have morphophysiological dormancy and low germination rates under natural conditions. The aim of the present study was to identify the genes involved in dormancy maintenance. In this study, the germination percentage of M. sieboldii seeds negatively correlated with the content of endogenous abscisic acid (ABA). The hydration of seeds for germination showed three distinct phases. Five key time points were identified: 0 h imbibition (dry seed, GZ), 0 day after imbibition (DAI), 16 DAI, 40 DAI, and 56 DAI. The comprehensive transcript profiles of M. sieboldii seeds treated with ABA and water at the five key germinating stages were obtained. A total of 9641 differentially expressed genes (DEGs) were identified, and 208 and 197 common DEGs were found throughout the ABA and water treatments, respectively. Compared with that in the GZ, 518, 696, 2133, and 1535 DEGs were identified in the SH group at 0, 16, 40 and 56 DAI, respectively. 666, 1725, 1560 and 1415 DEGs were identified in the ABA group at 0, 16, 40, and 56 DAI, respectively. Among the identified DEGs, 12 722 were annotated with GO terms, the top three enriched GO terms were different among the DEGs at 56 DAI in the ABA vs. SH treatments. KEGG pathway enrichment analysis for DEGs indicated that oxidative phosphorylation, protein processing in endoplasmic reticulum, starch and sucrose metabolism play an important role in seed response to ABA. 1926 TFs are obtained and classified into 72 families from the M. sieboldii transcriptome. Results of differential gene expression analysis together with qRT-PCR indicated that phase II is crucial for rapid and successful seed germination. This study is the first to present the global expression patterns of ABA-regulated transcripts in M. sieboldii seeds at different germinating phases.

Keywords: Abscisic acid; Germination; Magnolia sieboldii; Transcriptome; Water content.

MeSH terms

Abscisic Acid* / metabolism
Abscisic Acid* / pharmacology
Gene Expression Profiling
Gene Expression Regulation, Plant
Germination / genetics
Humans
Magnolia* / genetics
Plant Dormancy / genetics
Seeds / metabolism
Transcriptome

Substances

Abscisic Acid