Enhanced pathogenicity by up-regulation of A20 after avian leukemia subgroup a virus infection

Xueyang Chen; Xingming Wang; Yuxin Yang; Chun Fang; Jing Liu; Xiongyan Liang; Yuying Yang

doi:10.3389/fvets.2022.1031480

Enhanced pathogenicity by up-regulation of A20 after avian leukemia subgroup a virus infection

Front Vet Sci. 2022 Nov 14:9:1031480. doi: 10.3389/fvets.2022.1031480. eCollection 2022.

Authors

Xueyang Chen^{1

2}, Xingming Wang¹, Yuxin Yang¹, Chun Fang¹, Jing Liu¹, Xiongyan Liang¹, Yuying Yang¹

Affiliations

¹ College of Animal Science, Yangtze University, Jingzhou, China.
² College of Agriculture, Yangtze University, Jingzhou, China.

Abstract

Avian leukemia virus subgroup A (ALV-A) infection slows chicken growth, immunosuppression, and tumor occurrence, causing economic loss to the poultry industry. According to previous findings, A20 has a dual role in promoting and inhibiting tumor formation but has rarely been studied in avians. In this study, A20 overexpression and shRNA interference recombinant adenoviruses were constructed and inoculated into chicken embryos, and ALV-A (rHB2015012) was inoculated into 1-day-old chicks. Analysis of body weight, organ index, detoxification, antibody production, organ toxin load, and Pathological observation revealed that A20 overexpression could enhance ALV-A pathogenicity. This study lays the foundation for subsequent exploration of the A20-mediated tumorigenic mechanism of ALV-A.

Keywords: A20; ALV-A; chicken; pathogenic; recombinant adenovirus.