Single-cell RNA-Seq analysis of diabetic wound macrophages in STZ-induced mice

Jiaxu Ma; Ru Song; Chunyan Liu; Guoqi Cao; Guang Zhang; Zhenjie Wu; Huayu Zhang; Rui Sun; Aoyu Chen; Yibing Wang; Siyuan Yin

doi:10.1007/s12079-022-00707-w

Single-cell RNA-Seq analysis of diabetic wound macrophages in STZ-induced mice

J Cell Commun Signal. 2023 Mar;17(1):103-120. doi: 10.1007/s12079-022-00707-w. Epub 2022 Nov 29.

Authors

Jiaxu Ma^{1

2}, Ru Song^{1

2}, Chunyan Liu^{3

2}, Guoqi Cao^{1

2}, Guang Zhang^{1

2}, Zhenjie Wu^{1

2}, Huayu Zhang^{1

2}, Rui Sun^{1

2}, Aoyu Chen^{3

2}, Yibing Wang^{4

5

6}, Siyuan Yin^{3

2}

Affiliations

¹ Department of Plastic Surgery, Cheeloo College of Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, 250012, Jinan, Shandong, P. R. China.
² Jinan Clinical Research Center for Tissue Engineering Skin Regeneration and Wound Repair, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, 250014, Jinan, P. R. China.
³ Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, The First Affiliated Hospital of Shandong First Medical University, 250014, Jinan, Shandong, P. R. China.
⁴ Department of Plastic Surgery, Cheeloo College of Medicine, Shandong Provincial Qianfoshan Hospital, Shandong University, 250012, Jinan, Shandong, P. R. China. ybwang@sdfmu.edu.cn.
⁵ Department of Plastic Surgery, Shandong Provincial Qianfoshan Hospital, The First Affiliated Hospital of Shandong First Medical University, 250014, Jinan, Shandong, P. R. China. ybwang@sdfmu.edu.cn.
⁶ Jinan Clinical Research Center for Tissue Engineering Skin Regeneration and Wound Repair, The First Affiliated Hospital of Shandong First Medical University & Shandong Provincial Qianfoshan Hospital, 250014, Jinan, P. R. China. ybwang@sdfmu.edu.cn.

Abstract

The crucial role of macrophages in the healing of chronic diabetic wounds is widely known, but previous in vitro classification and marker genes of macrophages may not be fully applicable to cells in the microenvironment of chronic wounds. The heterogeneity of macrophages was studied and classified at the single-cell level in a chronic wound model. We performed single-cell sequencing of CD45 + immune cells within the wound edge and obtained 17 clusters of cells, including 4 clusters of macrophages. One of these clusters is a previously undescribed population of macrophages possessing osteoclast gene expression, for which analysis of differential genes revealed possible functions. We also analysed the differences in gene expression between groups of macrophages in the control and diabetic wound groups at different sampling times. We described the differentiation profile of mononuclear macrophages, which has provided an important reference for the study of immune-related mechanisms in diabetic chronic wounds.

Keywords: Chronic diabetic wound.; Macrophage; Single-cell RNA sequencing.

Abstract

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