Slow hydrolysis of amylose in soluble starch and amylopectin in suspendable starch liberated from non-glutinous rice flour heated with a sorghum extract

Umeo Takahama; Toshihiro Ansai; Sachiko Hirota

doi:10.1016/j.heliyon.2022.e11605

Slow hydrolysis of amylose in soluble starch and amylopectin in suspendable starch liberated from non-glutinous rice flour heated with a sorghum extract

Heliyon. 2022 Nov 17;8(11):e11605. doi: 10.1016/j.heliyon.2022.e11605. eCollection 2022 Nov.

Authors

Umeo Takahama¹, Toshihiro Ansai², Sachiko Hirota³

Affiliations

¹ Emeritus Professor of Kyushu Dental University, Kitakyushu 803-8580, Japan.
² Division of Community Oral Health Development, Kyushu Dental University, Kitakyushu 803-8580, Japan.
³ Sanyo-Gakuen College, Okayama 703-8501, Japan.

Abstract

Polyphenols in plant can interact with amylose and amylopectin in different ways affecting their hydrolysis by α-amylase. Pancreatin liberated starch from non-glutinous rice flour heated with and without an aqueous extract of sorghum seeds, and hydrolyzed the liberated starch. The hydrolysis of the liberated starch was slowed down by the sorghum extract. Then, the liberated starch was fractionated into soluble starch and suspendable starch. In the soluble starch, amylose hydrolysis was slowed down more significantly than amylopectin hydrolysis, and in the suspendable starch, the hydrolysis of amylopectin was slowed down efficiently by the sorghum extract. It is discussed that (i) the slowdown in the former might be due to the binding of sorghum components including procyanidins to amylose, and that (ii) the slowdown in the latter might be due to the complex formation between amylopectin and shorter amylose combined with the sorghum components. The contribution of amylose to the slowdown was supported by the result that the sorghum extract inhibited the starch hydrolysis only slightly in glutinous rice flour, the starch of which was almost composed of amylopectin. It was proposed a possible mechanism of the slowdown of amylopectin hydrolysis in suspendable starch by shorter amylose combined with the sorghum components.

Keywords: Amylose/amylopectin hydrolysis; Formation of starch/procyanidin complexes; Fractionation of starch-iodine complexes; Pancreatin; Sorghum extract.