Vanadium (V) contamination of soils poses potential risks to humans and ecosystems. This study was conducted to evaluate the effects of endophyte-assisted phytoremediation and to determine the mechanisms involved in V detoxification and plant growth promotion. Results showed that the endophytic bacterium Serratia marcescens PRE01 could successfully colonize the roots and increase the total V uptake of Pteris vittata by 25.4 %, with higher plant biomass and V accumulation in roots. Endophyte inoculation significantly improved the secretion of phytic, malic, and oxalic acids and accelerated FeVO4 dissolution and subsequent Fe and V uptake in the rhizosphere. Under V stress without inoculation, V removed by shoot uptake, root uptake, and root surface adsorption accounted for 21.76 %, 42.14 %, and 30.93 % of the total V removal efficiency, respectively. To detoxify excess V, PRE01 effectively strengthened the adsorption of V on the root surface, with an increase in its contribution to the total V removal efficiency from 30.93 % to 38.10 %. Furthermore, beneficial endophytes could alleviate oxidative damage caused by V stress by reinforcing the plant antioxidant system and promoting V(V) reduction in root tissues. These findings clearly reveal that inoculation with endophytes is a promising method for modulating multiple strategies to enhance the phytoremediation of V-contaminated soils.
Keywords: Detoxification; Endophyte-assisted phytoremediation; Root exudates; Soil pollution; Vanadium.
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