Effects of co-modified biochar immobilized laccase on remediation and bacterial community of PAHs-contaminated soil

Zeqi Zheng; Weitao Liu; Qixing Zhou; Jiantao Li; Aurang Zeb; Qi Wang; Yuhang Lian; Ruiying Shi; Jianlin Wang

doi:10.1016/j.jhazmat.2022.130372

Effects of co-modified biochar immobilized laccase on remediation and bacterial community of PAHs-contaminated soil

J Hazard Mater. 2023 Feb 5;443(Pt B):130372. doi: 10.1016/j.jhazmat.2022.130372. Epub 2022 Nov 10.

Authors

Zeqi Zheng¹, Weitao Liu², Qixing Zhou¹, Jiantao Li¹, Aurang Zeb¹, Qi Wang¹, Yuhang Lian¹, Ruiying Shi¹, Jianlin Wang¹

Affiliations

¹ Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education (MOE), College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China; Tianjin Key Laboratory of Urban Ecology Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
² Key Laboratory of Pollution Processes and Environmental Criteria, Ministry of Education (MOE), College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China; Tianjin Key Laboratory of Urban Ecology Environmental Remediation and Pollution Control, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China. Electronic address: lwt@nankai.edu.cn.

PMID: 36444066
DOI: 10.1016/j.jhazmat.2022.130372

Abstract

Considering the stability and economy of immobilized enzymes, this study prepared co-modified biochar immobilized laccase product named Fe₃O₄@NaBC@GA@LC via orthogonal experimental design and explored its possibility of remediating polycyclic aromatic hydrocarbons (PAHs) contaminated soil in steel plants. Compared with the free laccase treatment, the relative activity of Fe₃O₄@NaBC@GA@LC remained 60 % after 50 days of incubation at room temperature. The relative activity of Fe₃O₄@NaBC@GA@LC could still retain nearly 80 % after five reuses. In the process of simulating the PAHs-contaminated site treatment experiment in Hangzhou Iron and steel plant, immobilized laccase exhibited efficient adsorption and degradation performances and even the removal rate of 5-ring PAHs reached more than 90 % in 40 days, resulting in improving urease activity and dehydrogenase in the soil and promoted the growth of a PAH degrading bacteria (Massilia). Our results further explained the efficient degradation effects of Fe₃O₄@NaBC@GA@LC on PAHs, which make it a promising candidate for PAHs-contaminated soil remediation.

Keywords: Bacterial community; Biochar; Immobilization; PAHs-contaminated soil; Stability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacteria
Laccase
Polycyclic Aromatic Hydrocarbons*
Soil
Steel

Substances

biochar
Polycyclic Aromatic Hydrocarbons
Laccase
Soil
Steel