Large-scale expanded sample imaging with tiling lattice lightsheet microscopy

Chieh-Han Lu; Cheng-Yu Huang; Xuejiao Tian; Peilin Chen; Bi-Chang Chen

doi:10.1016/j.biocel.2022.106340

Large-scale expanded sample imaging with tiling lattice lightsheet microscopy

Int J Biochem Cell Biol. 2023 Jan:154:106340. doi: 10.1016/j.biocel.2022.106340. Epub 2022 Nov 26.

Authors

Chieh-Han Lu¹, Cheng-Yu Huang², Xuejiao Tian³, Peilin Chen⁴, Bi-Chang Chen²

Affiliations

¹ Research Center for Applied Sciences, Academia Sinica, Taipei 115, Taiwan; Institute and Undergraduate Program of Electro-Optical Engineering, National Taiwan Normal University, Taipei 116, Taiwan. Electronic address: chenb10@gate.sinica.edu.tw.
² Research Center for Applied Sciences, Academia Sinica, Taipei 115, Taiwan.
³ Research Center for Applied Sciences, Academia Sinica, Taipei 115, Taiwan; Nano Science and Technology Program, Taiwan International Graduate Program, Academia Sinica, Taipei 115, Taiwan; Department of Engineering and System Science, National Tsing Hua University, Hsinchu 300, Taiwan.
⁴ Research Center for Applied Sciences, Academia Sinica, Taipei 115, Taiwan; Institute of Physics, Academia Sinica, Taipei 115, Taiwan.

PMID: 36442734
DOI: 10.1016/j.biocel.2022.106340

Abstract

The ability to observe biological nanostructures forms a vital step in understanding their functions. Thanks to the invention of expansion microscopy (ExM) technology, super-resolution features of biological samples can now be easily visualized with conventional light microscopies. However, when the sample is physically expanded, the demand for deep and precise 3D imaging increases. Lattice lightsheet microscopy (LLSM), which utilizes a planar illumination that is confined within the imaging depth of high numerical aperture (NA=1.1) detection objective, fulfils such requirements. In addition, optical tiling could be implemented to increase the field of view (FoV) by moving the lightsheet without mechanically moving the samples or the objective for high-precision 3D imaging. In this review article, we will explain the principle of the tiling lattice lightsheet microscopy (tLLSM), which combines optical tiling and lattice lightsheet, and discuss the applications of tLLSM in ExM.

Keywords: 3D imaging; Expansion microscopy; Lightsheet microscopy; Super-resolution imaging; Thick tissue imaging; Tiling lattice lightsheet.

Publication types

Review
Research Support, Non-U.S. Gov't

MeSH terms

Microscopy* / methods