Potential role of long noncoding RNA RP5-881L22.5 as a novel biomarker and therapeutic target of colorectal cancer

Hua Zong; Jian-Qiang Zou; Jian-Peng Huang; Shi-Ting Huang

doi:10.4251/wjgo.v14.i11.2108

Potential role of long noncoding RNA RP5-881L22.5 as a novel biomarker and therapeutic target of colorectal cancer

World J Gastrointest Oncol. 2022 Nov 15;14(11):2108-2121. doi: 10.4251/wjgo.v14.i11.2108.

Authors

Hua Zong¹, Jian-Qiang Zou², Jian-Peng Huang², Shi-Ting Huang²

Affiliations

¹ Department of Gastrointestinal Surgery, The Third People's Hospital of Shenzhen, Shenzhen 518000, Guangdong Province, China. zonghua@mail.sustech.edu.cn.
² Department of Gastrointestinal Surgery, The Third People's Hospital of Shenzhen, Shenzhen 518000, Guangdong Province, China.

Abstract

Background: The incidence of colorectal cancer in humans is high, and it is in the top five for cancer-related morbidity and mortality. It is one of the main threats to human health. The function of long noncoding RNAs in tumor occurrence and development has gradually gained attention in recent years. In increasing numbers of studies, researchers have demonstrated that it plays an important role in the pathogenesis of colorectal cancer.

Aim: To find out if long noncoding RNA RP5-881L22.5 played a role in the pathogenesis of colorectal cancer in relation to the tumor microenvironment.

Methods: We analyzed the transcriptome data and clinical data in The Cancer Genome Atlas-colon adenocarcinoma. The CIRBERSORT algorithm was applied to evaluate these tumor-infiltrating immune cells in The Cancer Genome Atlas-colon adenocarcinoma cancer tissue samples. Using the "estimate" package in R, we assessed the tumor immune microenvironment. The expression level of RP5-881L22.5 in tumor tissue and adjacent normal tissue samples from 4 pairs of colorectal cancer patients was determined by quantitative reverse transcription PCR. Colorectal cancer cells were tested for invasiveness using a transwell invasion assay after RP5-881L22.5 expression was knocked down.

Results: The expression of lncRNA RP5-881L22.5 was related to the clinical characteristics of the tumors, and it was negatively related to the infiltration level of immune cells in the tumor microenvironment and the expression of T cell inhibitory receptors. A major function of its coexpressed mRNA was to regulate tumor immunity, such as the immune response. When quantitative reverse transcription PCR was performed on tumor tissues from 4 pairs of colorectal cancer patients, the results showed that RP5-881L22.5 was highly expressed. Subsequently, knocking down the expression of RP5-881L22.5, the invasiveness of colorectal cancer cell lines was reduced, and the apoptosis rate was increased.

Conclusion: RP5-881L22.5 plays a crucial role in the microenvironment of tumors as well as in the pathogenesis of colorectal cancer. The relationship between RP5-881L22.5 and the tumor immune microenvironment deserves further study.

Keywords: Biomarker; Colorectal cancer; Long noncoding RNA RP5-881L22.5; Therapeutic target; Tumor immune microenvironment.