Free lipid A and full-length lipopolysaccharide coexist in Vibrio parahaemolyticus ATCC33846

Danyang Huang; Fan Ji; Xin Tan; Jun Qiao; Hedan Li; Zhen Wang; Xiaoyuan Wang

doi:10.1016/j.micpath.2022.105889

Free lipid A and full-length lipopolysaccharide coexist in Vibrio parahaemolyticus ATCC33846

Microb Pathog. 2023 Jan:174:105889. doi: 10.1016/j.micpath.2022.105889. Epub 2022 Nov 23.

Authors

Danyang Huang¹, Fan Ji², Xin Tan¹, Jun Qiao², Hedan Li², Zhen Wang², Xiaoyuan Wang³

Affiliations

¹ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
² State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China.
³ State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 214122, China; International Joint Laboratory on Food Safety, Jiangnan University, Wuxi, 214122, China; School of Food Science and Technology, Jiangnan University, Wuxi, 214122, China. Electronic address: xwang@jiangnan.edu.cn.

PMID: 36435436
DOI: 10.1016/j.micpath.2022.105889

Abstract

Lipid A plays an important role in the pathogenicity and antimicrobial resistance of Vibrio parahaemolyticus, but little is known about the structure and biosynthesis of lipid A in V. parahaemolyticus. In this study, lipid A species were either directly extracted or obtained by the acid hydrolysis of lipopolysaccharide from V. parahaemolyticus ATCC33846 cells and analyzed by thin-layer chromatography and high-performance liquid chromatography-tandem mass spectrometry. Several lipid A species in V. parahaemolyticus cells were characterized, and two of these species were not connected to polysaccharides. One free lipid A species has the similar structure as the hexa-acylated lipid A in Escherichia coli, and the other is a hepta-acylated lipid A with an additional secondary C16:0 acyl chain. Three lipid A species were isolated by the acid hydrolysis of lipopolysaccharide: the 1^st one has the similar structure as the hexa-acylated lipid A in E. coli, the 2^nd one is a hepta-acylated lipid A with an additional secondary C16:0 acyl chain and a secondary 2-OH C12:0 acyl chain, and the 3^rd one is equal to the 2^nd species with a phosphoethanolamine modification. These results are important for understanding the biosynthesis of lipid A in V. parahaemolyticus.

Keywords: Free lipid A; Lipopolysaccharide; Phosphoethanolamine; Secondary acylation; Vibrio parahaemolyticus.

MeSH terms

Escherichia coli
Lipid A / chemistry
Lipopolysaccharides*
Mass Spectrometry
Vibrio parahaemolyticus*

Substances

Lipopolysaccharides
Lipid A