In a previous study of the effects of intermittent extremely low frequency (ELF) magnetic fields (MF) on umbilical cord blood lymphocytes (UCBL), we evaluated MF amplitudes between 6 µT and 24 µT and found an effect only for those below 13 µT. This suggested the existence of an amplitude window. In this brief communication, we further tested this hypothesis. UCBLs from healthy newborns were isolated and exposed for 72 h to an intermittent ELF-MF (triangular, 7.8 Hz, 250 s ON/250 s OFF) with 6 different amplitudes between 3 µT and 12 µT, utilizing an oblong coil. Percentage of viable, early apoptotic (EA), and late apoptotic/necrotic (LAN) cells were determined by flow cytometry. Moreover, reactive oxygen species (ROS) were determined at 1 h and 3 h of the exposure. Like in our previous work, neither EA, nor LAN, nor ROS were statistically significantly affected by the intermittent ELF-MF. However, the percentage of viable cells was decreased by exposure to the fields with intensities of 6.5 µT and 12 µT (p < 0.05; and p = 0.057 for 8.5 µT). ELF-MF decreased the percentage of viable cells for fields down to 6.5 µT, but not for 5 µT, 4 µT, or 3 µT. Combined with our previous findings, the results reported here indicate an amplitude window effect between 6 µT and 13 µT. The obtained data are in line with a notion of amplitude and frequency windows, which request scanning of both amplitude and frequency while studying the ELF-MF effects.
Keywords: apoptosis; inhomogeneous magnetic fields; reactive oxygen species; viability.