Fabricated polyhydroxyalkanoates blend scaffolds enhance cell viability and cell proliferation

Abstract

For tissue engineering applications, cell adhesion and proliferation are crucial factors, and blending polymers is one of the most effective ways to create a biocompatible scaffold with desired properties. In order to create new potential porous, biodegradable scaffolds using salt leaching technique, poly(3-hydroxybutyrate) (PHB) and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) were blended in different ratios. SEM, BET, FTIR, and water contact angle measurements were used to analyze the scaffolds' porous surface, surface area, and roughness, chemical interaction, and hydrophilicity. Additionally, a hemolysis assay revealed that the mixtures were hemocompatible and had no impact on red blood cells. Different cells- Vero, Hela and MDBK cell lines cultured on the porous mats of these biopolymeric scaffolds exhibited significant increase in cell viability and attachment over time. The overall finding was that blended scaffolds exhibited reduced crystallinity, diverse porosity, higher surface area and hydrophilicity, and better cell viability, proliferation and adhesion. Our findings imply that a blended scaffold could be more suitable for use in tissue engineering applications.

Keywords: Cell adhesion and proliferation; Poly(3-hydroxybutyrate) (PHB); Poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV); Salt leaching; Scaffolds; Tissue engineering.