Novel hydrogel comprising non-ionic copolymer with various concentrations of pharmacologically active bile acids for cellular injectable gel

Bozica Kovacevic; Susbin Raj Wagle; Corina Mihaela Ionescu; Melissa Jones; Michael Lewkowicz; Elaine Ym Wong; Sanja Kojic; Goran Stojanovic; Maja Đanić; Momir Mikov; Armin Mooranian; Hani Al-Salami

doi:10.1016/j.colsurfb.2022.113014

Novel hydrogel comprising non-ionic copolymer with various concentrations of pharmacologically active bile acids for cellular injectable gel

Colloids Surf B Biointerfaces. 2023 Feb:222:113014. doi: 10.1016/j.colsurfb.2022.113014. Epub 2022 Nov 11.

Affiliations

¹ The Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, Australia; Hearing Therapeutics Department, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Nedlands, Perth, WA 6009, Australia.
² Hearing Therapeutics Department, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Nedlands, Perth, WA 6009, Australia.
³ Faculty of Technical Sciences, University of Novi Sad, Novi Sad, Serbia.
⁴ Department of Pharmacology, Toxicology and Clinical Pharmacology, Faculty of Medicine, University of Novi Sad, Novi Sad 21101, Serbia.
⁵ The Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, Australia; Hearing Therapeutics Department, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Nedlands, Perth, WA 6009, Australia; School of Pharmacy, University of Otago, Dunedin, Otago, New Zealand. Electronic address: a.mooranian@curtin.edu.au.
⁶ The Biotechnology and Drug Development Research Laboratory, Curtin Medical School & Curtin Health Innovation Research Institute, Curtin University, Bentley, Perth, WA 6102, Australia; Hearing Therapeutics Department, Ear Science Institute Australia, Queen Elizabeth II Medical Centre, Nedlands, Perth, WA 6009, Australia. Electronic address: hani.al-salami@curtin.edu.au.

PMID: 36427407
DOI: 10.1016/j.colsurfb.2022.113014

Abstract

Deoxycholic acid (DCA) is a bile acid capable of forming micelles and modifying the properties of hydrogels. We incorporated DCA in sodium alginate (SA) and poloxamer 407 matrices creating novel DCA-copolymer hydrogel for therapeutic delivery. Hydrogels were assessed for common rheological properties. Biocompatibility and biological effect were examined on various cell lines. Cell viability was determent in normal and various hypoxic conditions, and full mitochondrial bioenergetic parameters were assessed in cell lines in order to illustrate hydrogel effects on survival, and cell metabolic profile within the hydrogels. Obtained data suggest that a low dose of DCA in permeable, biocompatible hydrogels can be beneficial for cells to combat hypoxic conditions.

Keywords: Bile acids; Hydrogels; Hydrogels’; Injectable gels; Matrices; Secondary bile acids.

MeSH terms

Alginates / pharmacology
Cell Line
Hydrogels* / pharmacology
Micelles*
Poloxamer

Substances

Hydrogels
Micelles
Alginates
Poloxamer