Leaf wettability, anatomy and ultra-structure of Nothofagus antarctica and N. betuloides grown under a CO2 enriched atmosphere

Abstract

Increasing CO₂ air concentration may affect wettability, anatomy and ultra-structure of leaves of Patagonian forest species, evergreen and deciduous plants potentially responding differently to such CO₂ increases. In this study, we analysed the wettability, anatomy and ultra-structure of leaves of Nothofagus antarctica (deciduous) and N. betuloides (evergreen) grown under high CO₂ concentrations. Leaf wettability was affected by increasing CO₂, in different directions depending on species and leaf side. In both species, soluble cuticular lipid concentrations per unit leaf area raised with higher CO₂ levels. Stomatal parameters (density, size of guard cells and pores) showed different responses to CO₂ increasing depending on the species examined. In both species, leaf tissues showed a general trend to diminish with higher CO₂ concentration. Cuticle thickness was modified with higher CO₂ concentration in N. betuloides, but not in N. antarctica leaves. In both species, chloroplasts were often damaged with the increase in CO₂ concentration. Our results show that several surface and internal leaf parameters can be modified in association with an increase in atmospheric CO₂ concentration which may very among plant species.

Keywords: Climate change; Leaf anatomy; Patagonian forest surface free energy; Wettability.