Facile synthesis of Cu N-lauroyl sarcosinate nanozymes with laccase-mimicking activity and identification of toxicity effects for C. elegans

Fanwei Meng; Guofang Ma; Jiahe Qiu; Zhendong Fu; Jiaqing Yan; Lingping Wang

doi:10.1039/d2ra03759a

Facile synthesis of Cu N-lauroyl sarcosinate nanozymes with laccase-mimicking activity and identification of toxicity effects for C. elegans

RSC Adv. 2022 Nov 16;12(51):32898-32902. doi: 10.1039/d2ra03759a. eCollection 2022 Nov 15.

Authors

Fanwei Meng^{1

2

3}, Guofang Ma¹, Jiahe Qiu¹, Zhendong Fu¹, Jiaqing Yan², Lingping Wang^{1

3}

Affiliations

¹ Key Laboratory for Molecular Enzymology and Engineering, The Ministry of Education, Jilin University Changchun 130012 China wanglp@jlu.edu.cn yjq@mail.jlu.edu.cn +86-431-8515-5348.
² Hospital of Stomatology, Jilin Universtiy Changchun 130012 PR China.
³ School of Life Sciences, Jilin University Changchun 130012 China.

Abstract

Nanozyme is a material with enzyme-like catalytic activity, which has been widely used in environmental, antibacterial, and other fields of research. However, there are few reports on the toxicity of nanozymes. In this work, nanozymes co-assembled from sodium N-lauroyl sarcosinate (Ls) and Cu ions possess a Cu(i)-Cu(ii) electron transfer system similar to that of natural laccases. Reaction kinetic studies show that the catalyst follows a typical Michaelis-Menten model. Cu-N-lauroyl sarcosinate nanozyme (Cu-Ls NZ) possess excellent laccase-like activity to oxidize a variety of phenol-containing substrates, such as phenol, 4-iodophenol, and 2,4,5-trichlorophenol. To evaluate the toxicity of the material, the nematode C. elegans was exposed to various concentrations of Cu-Ls NZ. Effects on physiological levels were determined. The results showed that high doses of Cu-Ls NZ increased the amount of reactive oxygen species (ROS), decreased the locomotor activity of nematodes, and inhibited their larval growth.