Signal sequence contributes to the immunogenicity of Pasteurella multocida lipoprotein E

Li-Ting Cheng; Chun-Yen Chu; Hung Vu-Khac; Thu-Dung Doan

doi:10.1016/j.psj.2022.102200

Signal sequence contributes to the immunogenicity of Pasteurella multocida lipoprotein E

Poult Sci. 2023 Jan;102(1):102200. doi: 10.1016/j.psj.2022.102200. Epub 2022 Sep 28.

Authors

Li-Ting Cheng¹, Chun-Yen Chu¹, Hung Vu-Khac², Thu-Dung Doan³

Affiliations

¹ Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Taiwan; International Degree Program in Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan.
² Institute of Veterinary Research and Development of Central Vietnam, Vietnam.
³ Graduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Taiwan; International Degree Program in Animal Vaccine Technology, International College, National Pingtung University of Science and Technology, Pingtung, Taiwan; General Research Service Center, National Pingtung University of Science and Technology, Taiwan. Electronic address: dung50biotech@gmail.com.

Abstract

Recombinant Pasterurella multocida lipoprotein E (PlpE) has been shown to protect against fowl cholera. This study aimed to determine if the signal sequence may contribute to the antigenicity and protective efficacy of recombinant PlpE. A small antigenic domain of PlpE (termed truncated PlpE, tPlpE) was constructed with (SP-tPlpE) or without (tPlpE) the signal sequence and evaluated in vitro and in vivo. In vitro, the HEK-Bule hTLR2 Cells were used to evaluate the activation of NF-kB in the test associated with the stimulation of the SP-tPlpE and tPlpE proteins. When chickens were immunized, compared to the tPlpE vaccine group, the SP-tPlpE group showed higher antibody levels and enhanced CD4⁺ T cell response. In a challenge test, the SP-tPlpE group showed a survival rate of 87.5% (n = 8), compared to 25% for the tPlpE group. It is confirmed that the inclusion of the native signal sequence enhanced protective efficacy against fowl cholera and may act as a vaccine adjuvant. The short SP-tPlpE construct is amenable to further vaccine engineering and has potential to be developed as a fowl cholera vaccine.

Keywords: fowl cholera; lipid moiety; lipoprotein E; signal sequence; subunit vaccine.

MeSH terms

Animals
Bacterial Outer Membrane Proteins
Bacterial Vaccines
Chickens
Cholera* / veterinary
Lipoproteins
Pasteurella Infections* / prevention & control
Pasteurella Infections* / veterinary
Pasteurella multocida*
Poultry Diseases* / prevention & control
Protein Sorting Signals

Substances

Protein Sorting Signals
Bacterial Outer Membrane Proteins
Bacterial Vaccines
Lipoproteins