In-vitro cell migration enhancing and pro-angiogenic active secondary metabolites from Jeffreycia zeylanica (L.) H. Rob., S.C. Keeley & Skvarla (Asteraceae)

W M Priyantha Samarasinghe; Chandani Ranasinghe; K Hector Jayawardana; Seneviratnage Somaratne; G M Kamal B Gunaherath

doi:10.1080/14786419.2022.2149515

In-vitro cell migration enhancing and pro-angiogenic active secondary metabolites from Jeffreycia zeylanica (L.) H. Rob., S.C. Keeley & Skvarla (Asteraceae)

Nat Prod Res. 2023 Nov-Dec;37(22):3821-3825. doi: 10.1080/14786419.2022.2149515. Epub 2022 Nov 24.

Authors

W M Priyantha Samarasinghe¹, Chandani Ranasinghe¹, K Hector Jayawardana², Seneviratnage Somaratne³, G M Kamal B Gunaherath¹

Affiliations

¹ Department of Chemistry, The Open University of Sri Lanka, Nugegoda, Sri Lanka.
² Department of Zoology, The Open University of Sri Lanka, Nugegoda, Sri Lanka.
³ Department of Botany, The Open University of Sri Lanka, Nugegoda, Sri Lanka.

PMID: 36423344
DOI: 10.1080/14786419.2022.2149515

Abstract

Jeffreycia zeylanica (L.) H. Rob., S. C. Keeley & Skvarla is used for the treatment of wounds in indigenous medicine practiced in Sri Lanka. The scratch wound assay (SWA) guided fractionation of hexanes extract of J. zeylanica led to the isolation of ethuliacoumarin (1), stigmasterol (2), β-amyrin (3) and lupeol (4) and a non-resolved triterpene alcohol mixture HF_5D1, all of which showed enhanced cell migration. The mixture HF_5D1 contained glut-5-en-3β-ol (5) and friedelin-3β-ol (6). The identities of compounds 1-6 were established by the analysis of spectroscopic data and comparison of them with those reported. The compounds 1-4 and the non-resolved triterpene alcohol mixture, HF_5D1 also exhibited significant proangiogenic response in chorioallantoic membrane (CAM) assay in addition to the enhanced cell migration. This is the first report of the occurrence of the compounds 1, 2, 4 and 5 in this plant.

Keywords: Asteraceae; Jeffreycia zeylanica (=Vernonia zeylanica); chorioallantoic membrane (CAM) assay; ethuliacoumarin; scratch wound assay (SWA); wound healing activity.