Proximity-Dependent In Vivo Biotin Labeling for Interactome Mapping in Marchantia polymorpha

Methods Mol Biol. 2023:2581:295-308. doi: 10.1007/978-1-0716-2784-6_21.

Abstract

Weak or transient protein-protein interactions (PPIs) are involved in a manifold of cellular processes in all living organisms, including plants. However, many of these interactions may remain undiscovered by co-immunoprecipitation (Co-IP) approaches due to their low binding affinities or transitory nature. Enzyme-mediated proximity-dependent in vivo biotin labeling can be a powerful strategy to efficiently capture weak and transient PPIs and has been successfully applied in different model angiosperm species. Here, we provide an optimized and robust protocol for biotin ligase-mediated proximity labeling for interactome mapping in the model liverwort Marchantia polymorpha.

Keywords: Interactomics; Marchantia polymorpha; Proximity labeling; miniTurbo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biotin
  • Biotinylation
  • Marchantia* / genetics

Substances

  • Biotin