Identification of Differentially Expressed Intronic Transcripts in Osteosarcoma

Emel Rothzerg; Jiake Xu; David Wood

doi:10.3390/ncrna8060073

Identification of Differentially Expressed Intronic Transcripts in Osteosarcoma

Noncoding RNA. 2022 Oct 25;8(6):73. doi: 10.3390/ncrna8060073.

Authors

Emel Rothzerg¹, Jiake Xu¹, David Wood²

Affiliations

¹ School of Biomedical Sciences, The University of Western Australia, Perth, WA 6009, Australia.
² Medical School, The University of Western Australia, Perth, WA 6009, Australia.

Abstract

Over the past decade; the discovery and characterization of long noncoding RNAs (lncRNAs) have revealed that they play a major role in the development of various diseases; including cancer. Intronic transcripts are one of the most fascinating lncRNAs that are located within intron regions of protein-coding genes, which have the advantage of encoding micropeptides. There have been several studies looking at intronic transcript expression profiles in cancer; but almost none in osteosarcoma. To overcome this problem; we have investigated differentially expressed intronic transcripts between osteosarcoma and normal bone tissues. The results highlighted that NRG1-IT1; FGF14-IT1; and HAO2-IT1 were downregulated; whereas ER3-IT1; SND1-IT1; ANKRD44-IT1; AGAP1-IT1; DIP2A-IT1; LMO7DN-IT1; SLIT2-IT1; RNF216-IT1; and TCF7L1-IT1 were upregulated in osteosarcoma tissues compared to normal bone tissues. Furthermore, we identified if the transcripts encode micropeptides and the transcripts' locations in a cell.

Keywords: differential expression; intronic transcript; lncRNA; micropeptide; osteosarcoma.

Grants and funding

This research was funded by the Abbie Basson Sarcoma Foundation (Sock it to Sarcoma!). E.R. was also supported by the Australian Graduate Women—Barbara Hale Fellowship.