Optimization of acquisition and processing times for the measurement of 1H to 13C polarization transfer kinetics

X Falourd; M Lahaye; C Rondeau-Mouro

doi:10.1016/j.mex.2022.101914

Optimization of acquisition and processing times for the measurement of ¹H to ¹³C polarization transfer kinetics

MethodsX. 2022 Nov 9:9:101914. doi: 10.1016/j.mex.2022.101914. eCollection 2022.

Authors

X Falourd^{1

2}, M Lahaye¹, C Rondeau-Mouro³

Affiliations

¹ INRAE, UR1268 BIA, F-44316, Nantes, France.
² INRAE, BIBS Facility, F-44316, Nantes, France.
³ INRAE, UR1466 OPAALE, 17 Avenue de Cucillé, CS 64427, F-35044 Rennes, France.

Abstract

Solid-state NMR (ssNMR) is a unique technique allowing the study of macromolecular assemblies in their native state without prior modification. The proposed method simplifies the current long and tedious data-acquisition and processing protocols for Variable Contact Time (VCT) ssNMR experiments used in dynamic studies of macromolecular assemblies. Using cellulose nanocrystals as a model for polysaccharide assembly, the acquisition time was reduced by decreasing the number of scans and shortening the recycling time required for the ¹H to ¹³C polarization transfers.•A tenfold reduction in the acquisition time for each kinetic point was achieved while maintaining a good signal to noise ratio.•The processing time for the pseudo-2D solid-state NMR data was also shortened by using signal intensities derived from peak picking rather than the classically-used spectral deconvolution method.•These optimizations enabled the molecular dynamic parameters T_1ρ ^H, T_HH, T_CH to be accessed within a day.

Keywords: Cellulose; Solid-state NMR; VCT-CPMAS.