Shenhuang plaster ameliorates the Inflammation of postoperative ileus through inhibiting PI3K/Akt/NF-κB pathway

Ting Liu; Min Xu; Zhenyu Shi; Meiya Li; Rongyun Wang; Yanan Shi; Xiao Xu; Tiejuan Shao; Qiuhua Sun

doi:10.1016/j.biopha.2022.113922

Shenhuang plaster ameliorates the Inflammation of postoperative ileus through inhibiting PI3K/Akt/NF-κB pathway

Biomed Pharmacother. 2022 Dec:156:113922. doi: 10.1016/j.biopha.2022.113922. Epub 2022 Oct 29.

Authors

Ting Liu¹, Min Xu², Zhenyu Shi³, Meiya Li⁴, Rongyun Wang⁵, Yanan Shi¹, Xiao Xu¹, Tiejuan Shao⁶, Qiuhua Sun⁷

Affiliations

¹ The College of Nursing, Zhejiang Chinese Medical University, Hangzhou, China.
² The College of Nursing, Zhejiang Chinese Medical University, Hangzhou, China; Department of Administration, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
³ Department of Orthopaedic Surgery, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou, China.
⁴ Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
⁵ The College of Nursing, Zhejiang Chinese Medical University, Hangzhou, China; School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
⁶ School of Basic Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, China.
⁷ The College of Nursing, Zhejiang Chinese Medical University, Hangzhou, China. Electronic address: sunqiuhua@zcmu.edu.cn.

PMID: 36411615
DOI: 10.1016/j.biopha.2022.113922

Abstract

Background: Although Shenhuang plaster (SHP) from traditional Chinese medicine prescriptions, has the potential to promote the recovery progression of postoperative ileus (POI), the underlying mechanism remains elusive. Along these lines, in this work, both in vivo and in vitro studies were conducted to systematically explore the regulatory effect and mechanism of SHP on the inflammatory response of the intestinal basal layer in the POI model mice.

Methods: Intestinal manipulation in mice was utilized for the POI model. The impact of SHP in response to POI was evaluated by carrying fluorescein-labeled dextran, histomorphology, immunohistochemistry, in combination with flow cytometry analysis and transcriptome RNA sequencing in vivo. Besides, the cytotoxicity of the SHP treatment on RAW264.7 cells was detected by cell counting kit-8 (CCK-8), the biological effects were assessed by polymerase chain reaction (PCR) and the potential influences on the PI3K/Akt/NF-κB pathway were identified through detecting the expression levels of P85, AKT, IKK and P65 by western blot in vitro.

Results: The implementation of the SHP treatment could significantly reduce the expressions of interleukin (IL)- 1β and tumor necrosis factor (TNF)-α in the intestine, whereas the recovery of gastrointestinal motility is promoted. In addition, SHP can regulate the polarization of macrophages, indicating that the proportion of the M2 type is increased after the application of the SHP treatment. In addition, SHP inhibited the activity of PI3K/AKT/NF-κB signaling pathway-related proteins.

Conclusion: SHP can significantly ameliorate the inflammatory response of POI and at the same time promote the recovery of gastrointestinal motility. Its mechanism may be mediated by the polarization of macrophages through the PI3K/AKT/NF-κB signaling pathway.

Keywords: Anti-inflammation; Macrophage polarization; PI3K/Akt/NF-κB pathway; Postoperative ileus; Shenhuang plaster.

MeSH terms

Animals
Ileus* / drug therapy
Inflammation / drug therapy
Mice
NF-kappa B* / metabolism
Phosphatidylinositol 3-Kinases / metabolism
Proto-Oncogene Proteins c-akt / metabolism
Tumor Necrosis Factor-alpha / pharmacology

Substances

NF-kappa B
Proto-Oncogene Proteins c-akt
Phosphatidylinositol 3-Kinases
Tumor Necrosis Factor-alpha