Immobilizing copper in loess soil using microbial-induced carbonate precipitation: Insights from test tube experiments and one-dimensional soil columns

Abstract

Biomineralization as an alternative to traditional remediation measures has been widely applied to remediate copper (Cu)-contaminated sites due to its environmental-friendly nature. Immobilizing Cu is, however, a challenging task as it inevitably causes inactivation of ureolytic bacteria. In the present work, a series of test tube experiments were conducted to derive the relationships of Cu immobilization efficiency versus pH conditions. The Cu speciation transformation that is invisible in the test tube experiments was investigated via numerical simulations. Apart from that, the one-dimensional soil column tests, accompanied by the X-ray diffraction (XRD) and Raman spectroscopy analysis, mainly aimed not only to investigate the variations of Cu immobilization efficiency with the depth but to reveal the underlying mechanisms affecting the Cu immobilization efficiency. The results of the test tube experiments highlight the necessity of narrowing pH ranges to as close as 7 by introducing an appropriate bacterial inoculation proportion. The coordination adsorption of Cu, while performing the one-dimensional soil column tests, is encouraged by alkaline environments, which differs from the test tube experiments where Cu²⁺ is capsulized by carbonate precipitates to prevent their migration. The findings highlight the potential of applying the microbial-induced carbonate precipitation (MICP) technology to Cu-rich water bodies and Cu-contaminated sites remediation.

Keywords: Bacterial cementation solution; Biomineralization; Coordination adsorption; Copper metal; Immobilization efficiency.