Identification of resistance to Fusarium head blight and molecular cytogenetics of interspecific derivatives between wheat and Psathyrostachys huashanica

Chenchen Hou; Jing Han; Liangliang Zhang; Qiang Geng; Li Zhao; Shuhui Liu; Qunhui Yang; Xinhong Chen; Jun Wu

doi:10.1270/jsbbs.21089

Identification of resistance to Fusarium head blight and molecular cytogenetics of interspecific derivatives between wheat and Psathyrostachys huashanica

Breed Sci. 2022 Jun;72(3):213-221. doi: 10.1270/jsbbs.21089. Epub 2022 Jun 11.

Authors

Chenchen Hou¹, Jing Han¹, Liangliang Zhang¹, Qiang Geng¹, Li Zhao¹, Shuhui Liu¹, Qunhui Yang¹, Xinhong Chen¹, Jun Wu¹

Affiliation

¹ Shaanxi Key Laboratory of Plant Genetic Engineering Breeding, College of Agronomy, Northwest A&F University, Yangling, Shaanxi, 712100, China.

Abstract

Psathyrostachys huashanica is a relative of wheat (Triticum aestivum L.) with many disease resistance genes that can be used to improve wheat disease resistance. In order to enrich the germplasm resources available in wheat genetics and breeding, we assessed Fusarium head blight (FHB) resistance in 45 interspecific derivatives between wheat and Psathyrostachys huashanica during two years from 2017-2018. Two interspecific derivatives comprising, H-34-8-2-6-1 and H-24-3-1-5-19-1 were identified as FHB resistant lines. These two lines were examined based on their morphology and cytogenetics, as well as by genomic in situ hybridization (GISH), fluorescence in situ hybridization (FISH), molecular markers, and 660K genotyping array to determine their genetic construction. The results confirmed H-34-8-2-6-1 as a wheat-P. huashanica 1Ns long arm ditelosomic addition line and H-24-3-1-5-19-1 as a wheat-P. huashanica 2Ns substitution line. Assessments of the agronomic traits showed that H-34-8-2-6 had significantly higher kernel number per spike and self-fertility rate than parent 7182. In addition, compared with 7182, H-24-3-1-5-19-1 had a much lower plant height while the other agronomic traits were relatively similar. The two new lines are valuable germplasm materials for breeding FHB resistance in wheat.

Keywords: 660K genotyping array analysis; Fusarium head blight; Psathyrostachys huashanica; ditelosomic addition line; marker analysis; molecular cytogenetics; substitution line.