Development of chloroplast transformation and gene expression regulation technology in land plants

Yaqi An; Yue Wang; Xinwei Wang; Jianwei Xiao

doi:10.3389/fpls.2022.1037038

Development of chloroplast transformation and gene expression regulation technology in land plants

Front Plant Sci. 2022 Oct 27:13:1037038. doi: 10.3389/fpls.2022.1037038. eCollection 2022.

Authors

Yaqi An^{1

2

3}, Yue Wang^{1

2

3}, Xinwei Wang⁴, Jianwei Xiao^{1

2

3}

Affiliations

¹ College of Biological Sciences and Biotechnology, Beijing Forestry University, Beijing, China.
² Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, Beijing Forestry University, Beijing, China.
³ The Tree and Ornamental Plant Breeding and Biotechnology Laboratory of National Forestry and Grassland Administration, Beijing Forestry University, Beijing, China.
⁴ College of Agriculture and Forestry, Hebei North University, Zhangjiakou, China.

Abstract

Chloroplasts in land plants have their own small circular DNA that is presumed to have originated from cyanobacteria-related endosymbionts, and the chloroplast genome is an attractive target to improve photosynthetic ability and crop yield. However, to date, most transgenic or genetic engineering technologies for plants are restricted to manipulations of the nuclear genome. In this review, we provide a comprehensive overview of chloroplast genetic engineering and regulation of gene expression from the perspective of history and biology, focusing on current and latest methods. In addition, we suggest techniques that may regulate the chloroplast gene expression at the transcriptional or post-transcriptional level.

Keywords: CRISPR/Cas; aptamer; chloroplast genome; dPPR; gene expression; transformation.

Publication types

Review