Analysis of phellinus igniarius effects on gastric cancer cells by atomic force microscopy

Jia-He Wang; Jia-Jia Wang; Tuo-Yu Ju; Yu-Xi Huang; Li-Xin Yuan; Ying-Hui Luo; Yu-Juan Chen; Zuo-Bin Wang

doi:10.1016/j.micron.2022.103376

Analysis of phellinus igniarius effects on gastric cancer cells by atomic force microscopy

Micron. 2023 Jan:164:103376. doi: 10.1016/j.micron.2022.103376. Epub 2022 Nov 1.

Authors

Jia-He Wang¹, Jia-Jia Wang², Tuo-Yu Ju¹, Yu-Xi Huang², Li-Xin Yuan², Ying-Hui Luo¹, Yu-Juan Chen³, Zuo-Bin Wang⁴

Affiliations

¹ International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China; Minstry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China; Centre for Opto/Bio-Nano Measurement and Manufacturing, Zhongshan Institute of Changchun University of Science and Technology, Zhongshan 528437, China; JR3CN & IRAC, University of Bedfordshire, Luton LU1 3JU, UK.
² International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China; Minstry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China; JR3CN & IRAC, University of Bedfordshire, Luton LU1 3JU, UK.
³ International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China; Minstry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China; JR3CN & IRAC, University of Bedfordshire, Luton LU1 3JU, UK. Electronic address: chenyujuan@cust.edu.cn.
⁴ International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China; Minstry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun 130022, China; Centre for Opto/Bio-Nano Measurement and Manufacturing, Zhongshan Institute of Changchun University of Science and Technology, Zhongshan 528437, China; JR3CN & IRAC, University of Bedfordshire, Luton LU1 3JU, UK. Electronic address: wangz@cust.edu.cn.

PMID: 36395664
DOI: 10.1016/j.micron.2022.103376

Abstract

Gastric cancer is one of the common malignant tumors in the world, which originates from the gene mutation of human cells. In this work, an atomic force microscope was used to quantitatively detect the changes of multiple physical parameters such as the cell morphology, surface roughness, elasticity modulus and adhesion force before and after Phellinus linteus stimulation. The experimental results show that Phellinus linteus can change the shape of gastric cancer cells (SGC-7901) from flat to spherical, and increase their height and surface roughness values. The adhesion force of cells is reduced and the elasticity modulus is increased. But there are no significant differences in the morphology and mechanical properties of gastric epithelial cells (GES-1). The results indicate that Phellinus linteus has a high anticancer effect on the gastric cancer cells, but has less toxic side effects on the gastric epithelial cells. This work proves that Phellinus linteus can be used as a preferred anticancer drug for the treatment of gastric cancer cells.

Keywords: Adhesion force; Atomic force microscopy; Cell morphology; Elasticity modulus; Phellinus linteus.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Basidiomycota*
Humans
Microscopy, Atomic Force
Stomach Neoplasms* / drug therapy

Supplementary concepts

Tropicoporus linteus
Phellinus igniarius