Myokines derived from contracting skeletal muscle suppress anabolism in MCF7 breast cancer cells by inhibiting mTOR

Amanda R Davis; Chelsea G Goodenough; Kim C Westerlind; Robert Strange; John W Deaver; Patrick J Ryan; Steven E Riechman; James D Fluckey

doi:10.3389/fphys.2022.1033585

Myokines derived from contracting skeletal muscle suppress anabolism in MCF7 breast cancer cells by inhibiting mTOR

Front Physiol. 2022 Oct 21:13:1033585. doi: 10.3389/fphys.2022.1033585. eCollection 2022.

Authors

Amanda R Davis¹, Chelsea G Goodenough¹, Kim C Westerlind², Robert Strange², John W Deaver¹, Patrick J Ryan¹, Steven E Riechman¹, James D Fluckey¹

Affiliations

¹ Texas A&M University, School of Education and Human Development, College Station, TX, United States.
² University of Colorado Health Sciences Center, Denver, CO, United States.

Abstract

There is strong evidence that physical activity has a profound protective effect against multiple types of cancer. Here, we show that this effect may be mediated by factors released from skeletal muscle during simulated exercise, in situ, which suppress canonical anabolic signaling in breast cancer. We report attenuated growth of MCF7 breast cancer cells in the presence of a rodent-derived exercise conditioned perfusate, independent of prior exercise training. This reduction was concomitant with increased levels of DEPTOR protein and reduced mTOR activity.

Keywords: cell culture; electrical stimulation; myokines; protein synthesis; tumor suppression.