Complete genome sequence and characterization of four Decapod iridescent virus 1 isolates from crab and shrimp

Pan Qin; Gengshen Wang; Yingjia Luan; Jianjun Xie; Jie He; Hui Shi; Wenjun Xu

doi:10.1016/j.jip.2022.107852

Complete genome sequence and characterization of four Decapod iridescent virus 1 isolates from crab and shrimp

J Invertebr Pathol. 2023 Feb:196:107852. doi: 10.1016/j.jip.2022.107852. Epub 2022 Nov 13.

Authors

Pan Qin¹, Gengshen Wang², Yingjia Luan¹, Jianjun Xie², Jie He², Hui Shi³, Wenjun Xu⁴

Affiliations

¹ Key Laboratory of Marine Biotechnology of Fujian Province, College of Marine Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China.
² Key Laboratory of Mariculture & Enhancement of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan 316100, China; Marine and Fisheries Research Institute, Zhejiang Ocean University, Zhoushan 316100, China.
³ Key Laboratory of Mariculture & Enhancement of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan 316100, China; Marine and Fisheries Research Institute, Zhejiang Ocean University, Zhoushan 316100, China. Electronic address: huishi2002@126.com.
⁴ Key Laboratory of Mariculture & Enhancement of Zhejiang Province, Zhejiang Marine Fisheries Research Institute, Zhoushan 316100, China; Marine and Fisheries Research Institute, Zhejiang Ocean University, Zhoushan 316100, China. Electronic address: wjxu1971@hotmail.com.

PMID: 36384189
DOI: 10.1016/j.jip.2022.107852

Abstract

Decapod iridescent virus 1 (DIV1) is an emerging viral pathogen that infects diverse freshwater and marine crustacean species and causes considerable economic losses that seriously threaten crustacean farming and has caused enormous financial losses in recent years. In this study, we detected DIV1 from diseased crabs, with clinical symptoms such as loss of vitality and white gill filaments with edema, in a Marsupenaeus japonicus and Portunus trituberculatus polyculture pond. Four DIV1 isolates from crab samples (two isolates) and shrimp samples (two isolates) were sequenced and assembled successfully. Molecular characterization and phylogenetic analysis of the four DIV1 isolates were conducted. The DIV1 isolates from crab samples have a close genetic relationship with shrimp DIV1s, indicating the viruses share the same ancestor with those from shrimps. Our study provides valuable insights into disease prevention and control of the shrimp-crab polyculture system.

Keywords: Crab; Decapod iridescent virus 1; Phylogenetic analysis; Shrimp.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brachyura*
Decapoda*
Penaeidae*
Phylogeny
Seafood