miR-155-1 as a positive factor for novel duck reovirus replication by regulating SOCS5-mediated interferons

Yu Dong; Hui Yan; Jinman Li; Lei Bei; Xingxing Shi; Yanli Zhu; Zhijin Xie; Ruihua Zhang; Shijin Jiang

doi:10.1016/j.virusres.2022.199003

miR-155-1 as a positive factor for novel duck reovirus replication by regulating SOCS5-mediated interferons

Virus Res. 2023 Jan 2:323:199003. doi: 10.1016/j.virusres.2022.199003. Epub 2022 Nov 13.

Authors

Yu Dong¹, Hui Yan¹, Jinman Li¹, Lei Bei¹, Xingxing Shi¹, Yanli Zhu¹, Zhijin Xie¹, Ruihua Zhang², Shijin Jiang³

Affiliations

¹ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, China.
² Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, China. Electronic address: rhzhang@sdau.edu.cn.
³ Department of Preventive Veterinary Medicine, College of Veterinary Medicine, Shandong Agricultural University, Tai'an, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Tai'an, China. Electronic address: sjjiang@sdau.edu.cn.

Abstract

Diseases caused by novel duck reovirus (NDRV) have brought considerable economic losses to the poultry industry. MicroRNAs (miRNAs) have an impact on virus replication and antiviral immunity. However, the miRNA profile upon NDRV infection in duck embryo fibroblasts (DEFs) remains to be discovered. In this study, small RNA (sRNA) sequencing was performed to decipher the cellular miRNA response to NDRV infection. Based on 26 differentially expressed miRNAs (19 upregulated and 7 downregulated miRNAs) obtained from sequencing data and their target genes predicted by software, GO and KEGG analyses were performed to elucidate the functions of miRNAs in NDRV invasion, replication, and virus spread. "FoxO signaling pathway", "autophagy", and "Toll-like receptor signaling pathway" might participate in NDRV replication as revealed by KEGG enrichment analysis. The miR-155-1 sequence was found to be identical to rno-miR-155-5p and was sharply increased with the progression of NDRV infection. Moreover, NDRV-induced miR-155-1 could act as a positive factor for virus replication in DEFs, which inhibited type I interferon (IFN-I) production. Luciferase assay confirmed that miR-155-1 disturbed the abundance of suppressor of cytokine signaling (SOCS) 5 by targeting 3'-UTR. SOCS5, which is linked to increased IRF7 expression, restricts IFN expression and promotes NDRV replication in DEFs. Therefore, this study proposed that miR-155-1 was used by NDRV to restrict SOCS5 expression, attenuating the production of IFN-I and creating a favorable environment for virus replication.

Keywords: IFN-I; NDRV; SOCS5; miR-155-1; miRNA.