Ion Microscope Imaging Mass Spectrometry Using a Timepix3-Based Optical Camera

J Am Soc Mass Spectrom. 2022 Dec 7;33(12):2328-2332. doi: 10.1021/jasms.2c00223. Epub 2022 Nov 16.

Abstract

Ion microscopy allows for high-throughput mass spectrometry imaging. In order to resolve congested mass spectra, a high degree of timing precision is required from the microscope detector. In this paper we present an ion microscope mass spectrometer that uses a Timepix3 hybrid pixel readout with an optimal 1.56 ns resolution. A novel triggering technique is also employed to remove the need for an external time-to-digital converter (TDC) and allow the experiment to be performed using a low-cost and commercially available readout system. Results obtained from samples of rhodamine B demonstrate the application of multimass imaging sensors for microscope mass spectrometry imaging with high mass resolution.

Keywords: fast imaging sensor; ion microscope imaging; mass spectrometry imaging; stigmatic ion imaging.