Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems

Qianqian Qi; Yutong Zhang; Wei Xiong; Xingyu Liu; Shuangyu Cui; Xiaofang Ye; Kaisong Zhang; Tian Tian; Xiang Zhou

doi:10.1039/d2sc02635j

Norbornene-tetrazine ligation chemistry for controlling RNA-guided CRISPR systems

Chem Sci. 2022 Oct 20;13(42):12577-12587. doi: 10.1039/d2sc02635j. eCollection 2022 Nov 2.

Authors

Qianqian Qi¹, Yutong Zhang¹, Wei Xiong¹, Xingyu Liu¹, Shuangyu Cui¹, Xiaofang Ye¹, Kaisong Zhang¹, Tian Tian¹, Xiang Zhou¹

Affiliation

¹ Key Laboratory of Biomedical Polymers of Ministry of Education, College of Chemistry and Molecular Sciences, Hubei Province Key Laboratory of Allergy and Immunology, Wuhan University Wuhan 430072 Hubei China ttian@whu.edu.cn.

Abstract

In the present study, norbornene-tetrazine ligation chemistry is harnessed for developing clickable RNA switches in biological contexts. This RNA control strategy is explored with a variety of applications. We further demonstrate the application of RNA-based norbornene-tetrazine ligation chemistry for controlling CRISPR systems. Moreover, the manipulation of gene editing in human cells is accomplished.