Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients

Ayame Mikagi; Ryosuke Tashiro; Tomoo Inoue; Riki Anzawa; Akiho Imura; Takahiro Tanigawa; Tomohisa Ishida; Takashi Inoue; Kuniyasu Niizuma; Teiji Tominaga; Toyonobu Usuki

doi:10.1039/d2ra06009d

Isotope-dilution LC-MS/MS analysis of the elastin crosslinkers desmosine and isodesmosine in acute cerebral stroke patients

RSC Adv. 2022 Nov 7;12(49):31769-31777. doi: 10.1039/d2ra06009d. eCollection 2022 Nov 3.

Authors

Ayame Mikagi¹, Ryosuke Tashiro², Tomoo Inoue^{1

2

3}, Riki Anzawa¹, Akiho Imura¹, Takahiro Tanigawa¹, Tomohisa Ishida³, Takashi Inoue³, Kuniyasu Niizuma^{4

5}, Teiji Tominaga², Toyonobu Usuki¹

Affiliations

¹ Department of Materials and Life Sciences, Faculty of Science and Technology, Sophia University 7-1 Kioicho Chiyoda-ku Tokyo 102-8554 Japan t-usuki@sophia.ac.jp.
² Department of Neurosurgery, Tohoku University Graduate School of Medicine 1-1 Seiryo-machi Aoba-ku Sendai 980-8574 Japan tomoo49@gmail.com.
³ Department of Neurosurgery, Sendai Medical Center 2-11-12 Miyagino Miyagino-ku Sendai 983-8520 Japan.
⁴ Department of Neurosurgical Engineering and Translational Neuroscience, Graduate School of Biomedical Engineering, Tohoku University 2-1 Seiryo-machi, Aoba-ku Sendai 980-8575 Japan niizuma@nsg.med.tohoku.ac.jp.
⁵ Department of Neurosurgical Engineering and Translational Neuroscience, Tohoku University Graduate School of Medicine 2-1 Seiryo-machi, Aoba-ku Sendai 980-8575 Japan.

Abstract

Utilizing chemically synthesized an isotopically labeled internal standard, isodesmosine-¹³C₃,¹⁵N₁, an isotope-dilution LC-MS/MS method was established. Concentrations of desmosine and isodesmosine in plasma of acute cerebral stroke patients and healthy controls were determined. The concentration of desmosines was markedly higher in plasma from acute stroke patients compared with healthy controls. Desmosines are thus novel biomarkers for evaluating the extent of vascular injury after acute cerebral stroke.