Novel methods for the generation of genetically engineered animal models

Annelise Cassidy; Melda Onal; Stephane Pelletier

doi:10.1016/j.bone.2022.116612

Novel methods for the generation of genetically engineered animal models

Bone. 2023 Feb:167:116612. doi: 10.1016/j.bone.2022.116612. Epub 2022 Nov 13.

Authors

Annelise Cassidy¹, Melda Onal², Stephane Pelletier³

Affiliations

¹ Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA.
² Department of Physiology and Cell Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
³ Department of Medical and Molecular Genetics, Indiana University School of Medicine, Indiana University-Purdue University Indianapolis, Indianapolis, IN 46202, USA. Electronic address: spellet@iu.edu.

Abstract

Genetically modified mouse models have shaped our understanding of biological systems in both physiological and pathological conditions. For decades, mouse genome engineering has relied on transgenesis and spontaneous gene replacement in embryonic stem (ES) cells. While these technologies provided a wealth of knowledge, they remain imprecise and expensive to use. Recent advances in genome editing technologies such as the development of targetable nucleases, the improvement of delivery systems, and the simplification of targeting strategies now allow for the rapid, precise manipulation of the mouse genome. In this review article, we discuss novel methods and targeting strategies for the generation of mouse models for the study of bone and skeletal muscle biology.

Keywords: CRISPR; Genetic engineering; Genome editing; Musculoskeletal system.

Publication types

Review

MeSH terms

Animals
Animals, Genetically Modified
CRISPR-Cas Systems*
Gene Editing*
Genetic Engineering / methods
Genetic Therapy
Mice

Grants and funding

R21 AR076575/AR/NIAMS NIH HHS/United States