In this work, a self-supplied electron photoelectrochemical (PEC) biosensor for sensitive determination of Pb2+ was established by utilizing donor-acceptor (D-A)-type PTB7-Th (poly{4,8-bis[5-(2-ethylhexyl) thiophen-2-yl]benzo[1,2-b,4,5-b']dithiophene-2,6-diyl-alt-3-fluoro-2-[(2-ethylhexyl)carbonyl] thieno[3,4-b]-thiophene-4,6-diyl}) as a photoelectric material coupled with biotin as an efficient signal quencher. Impressively, compared with the traditional PEC signal quenchers, biotin was first applied as a PEC signal quencher in this work and it effectively avoided a cumbersome preparation process, complex DNA sequence design, and extra reagent assistance and greatly simplified experimental steps, which could achieve an efficient PEC signal quenching toward PTB7-Th. In addition, the execution of a DNAzyme-assisted Pb2+ recycling amplification reaction could release the quencher biotin, leading to the recovery of the PEC signal, thereby realizing the quantitative detection of Pb2+. Resultantly, the submitted self-supplied electron PEC biosensor presented an extensive coverage of assay Pb2+ (50 fM to 500 nM) along with a low determination limit (16.7 fM), which exhibited the advantages of high selectivity and excellent stability. Importantly, this work provided a powerful alternative to traditional heavy metal-ion assessment methods and possessed the potential for application in environment, biomedicine, and food-safety fields.
Keywords: PTB7-Th; biotin; lead ion; photoelectrochemical biosensor; quencher.